Molecular characterization of human calicivirus associated with acute diarrheal disease in Mexican children.

Virology Journal
Fabián Gómez-SantiagoHerlinda García-Lozano

Abstract

Human caliciviruses (HuCV) are emerging enteric pathogens that are a common cause of diarrhea in humans worldwide. Due to the paucity of information on the molecular characterization of HuCV circulating in Mexico, the aim of this work was to investigate the diversity and molecular epidemiology of the HuCV infection associated with acute diarrheal disease in Mexican children aged up to 5 years. Of the 131/414 (32%) HuCV positive-specimens analyzed, 128 were identified as Norovirus (NoV) and three as Sapovirus (SaV). Of the NoV positive specimens, 118/128 (92%) were NoV GII and 10/128(8%) were untypeable by RT-PCR in both polymerase and capsid genes, whereas one SaV isolate was further confirmed by sequencing as GI.2. Phylogenetic analysis based on polymerase partial gene sequences from 89/131 (68%) HuCV isolates showed that 86/89 (97%) belong to NoV GII.4 with three main variant clusters of this genotype, 2/89 (2%) to NoV GII.2, and 1/89 (1%) to SaV GI.2. Furthermore, partial sequencing of the capsid gene VP1 of 63/131 (48%) strains indicated that 61/63 (97%) correlated with NoV GII.4, whereas only 2/63 (3%) clustered to NoV GII.2. HuCV infections were detected throughout the year, and the highest number of cases positive for No...Continue Reading

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Citations

Apr 23, 2014·Molecular and Cellular Probes·I G A RuetherP Markoulatos
Dec 12, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·T N Hoa TranOsamu Nakagomi
Mar 29, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tatiane da Silva PolóAxel Mauroy
Apr 27, 2016·PLoS Pathogens·Johan NordgrenLennart Svensson
Jun 1, 2018·BMC Infectious Diseases·Kgomotso MakhaolaLemme P Kebaabetswe
Jun 8, 2017·Memórias do Instituto Oswaldo Cruz·Samya Thalita Picanço da CostaYvone Benchimol Gabbay
Oct 9, 2020·Food and Environmental Virology·Leticia Félix-ValenzuelaVerónica Mata-Haro

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Datasets Mentioned

BETA
AB039781
AF145896
AF182760
AJ011099
AJ249939
AY134748
AY228235
AY032605
AY485642
DQ078829

Methods Mentioned

BETA
Enzyme-linked immunosorbent assays
ELISA
genotyping
electrophoresis
Enzyme linked immunosorbent assay

Software Mentioned

MEGA
Basic Local Alignment Search Tool ( BLAST )
Basic Local Alignment Search Tool
Clustal W
BLAST
ChromasPro

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