Molecular characterization of IMP-type metallo-β-lactamases among multidrug-resistant Achromobacter xylosoxidans

The Journal of Antimicrobial Chemotherapy
Masaki YamamotoSatoshi Ichiyama

Abstract

To analyse the metallo-β-lactamase (MBL) genes and their genetic environments in clinical isolates of Achromobacter xylosoxidans. From January 2004 to December 2010, four MBL-producing, multidrug-resistant (MDR) A. xylosoxidans strains were isolated and analysed at a tertiary care university hospital in Japan. Species-level identification was confirmed by 16S ribosomal RNA gene sequencing. Molecular typing was performed using PFGE, the presence of MBL genes was detected using PCR, and integron gene cassettes were examined by cloning and sequence analysis of integron PCR products. The plasmids obtained from individual isolates were analysed based on EcoRI restriction patterns, Southern hybridizations using digoxigenin-labelled probes for bla(IMP-1) and bla(IMP-19) as well as conjugation and transformation experiments. No clonal relationship was found among the four A. xylosoxidans isolates. Three isolates harboured bla(IMP-1) and one isolate harboured bla(IMP-19). These MBL genes were carried on class 1 integrons. Four different class 1 integron gene cassette arrays were found, including orf1-bla(IMP-1)-aacA4, bla(IMP-1)-bla(IMP-1)-nit1/nit2-catB3-bla(IMP-1)-bla(IMP-1), aacA4-bla(IMP-1) and bla(IMP-19). The restriction pattern o...Continue Reading

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Aug 25, 2011·The Journal of Antimicrobial Chemotherapy·Masaki YamamotoSatoshi Ichiyama

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Citations

Nov 16, 2012·The Journal of Antimicrobial Chemotherapy·Simona PolliniGian Maria Rossolini
Jan 27, 2015·Foodborne Pathogens and Disease·Marjorie E Doyle
Jan 19, 2017·Emerging Infectious Diseases·Lucie AmoureuxCatherine Neuwirth
Dec 10, 2014·Antimicrobial Agents and Chemotherapy·Yongfei HuBaoli Zhu
Jan 29, 2014·Virology Journal·Johannes WittmannChristine Rohde
Nov 7, 2019·Critical Reviews in Microbiology·Pompeyo FerroCélia M Manaia
Aug 8, 2021·International Journal of Molecular Sciences·Fernando Sanz-GarcíaSara Hernando-Amado

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