PMID: 9422611Jan 9, 1998Paper

Molecular characterization of IS1541 insertions in the genome of Yersinia pestis

Journal of Bacteriology
M OdaertMichel Simonet

Abstract

The genome of Yersinia pestis, the causative agent of plague, contains at least 30 copies of an element, designated IS1541, which is structurally related to IS200 (85% identity). One such element is inserted within the chromosomal inv gene (M. Simonet, B. Riot, N. Fortineau, and P. Berche, Infect. Immun. 64:375-379, 1996). We characterized other IS1541 insertions by cloning 14 different Y. pestis 6/69M loci carrying a single copy of this insertion sequence (IS) into Escherichia coli and, for each element, sequencing 250 bp of both flanking regions. In no case was this IS element inserted into large open reading frames; however, in eight cases, it was detected downstream (17 to 139 bp) of genes thought to be transcribed monocistronically or which constituted the last gene of an operon, and in only one case was it detected upstream (37 bp) of the first gene of an operon. Sequence analysis revealed stem-loop structures (deltaG, < -10 kcal) resembling rho-independent transcription terminators in 8 of the 14 insertion sites. These motifs might constitute hot spots for insertion of this IS1541 element within the Y. pestis genome.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R M GemmillJ M Calvo
Apr 1, 1991·Molecular & General Genetics : MGG·K JahreisJ W Lengeler
Jul 1, 1991·Clinical Microbiology Reviews·R R Brubaker
Dec 1, 1990·Journal of General Microbiology·I GibertJ Casadesús
Nov 1, 1990·Journal of Bacteriology·C O'ReillyD J McConnell
Mar 11, 1990·Nucleic Acids Research·E Ricca, J M Calvo
Jan 20, 1986·Journal of Molecular Biology·S Lam, J R Roth
Oct 1, 1983·Cell·S Lam, J R Roth
Feb 1, 1981·Infection and Immunity·D M Ferber, R R Brubaker
Sep 1, 1994·FEMS Microbiology Letters·O N PodladchikovaJ Heesemann
Jan 1, 1995·Journal of Bacteriology·A MatsuhisaK Shiba
Mar 1, 1994·Journal of Clinical Microbiology·A GuiyouleE Carniel
Sep 1, 1996·Journal of Clinical Microbiology·M OdaertM Simonet
Jan 1, 1997·Clinical Microbiology Reviews·R D Perry, J D Fetherston

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Citations

Aug 10, 2002·Journal of Bacteriology·Caroline WildeJean-Marie Clément
Jul 27, 2001·Applied and Environmental Microbiology·L RadnedgeG L Andersen
Oct 23, 2004·Emerging Infectious Diseases·Michel DrancourtDidier Raoult
Aug 5, 2009·Briefings in Functional Genomics & Proteomics·Magali Naville, Daniel Gautheret
Jun 6, 2015·Nucleic Acids Research·Michael J EllisDavid B Haniford
Feb 25, 2009·Infection and Immunity·Claire A CorneliusOlaf Schneewind
Sep 16, 2005·The EMBO Journal·Bao Ton-HoangMichael Chandler
Sep 12, 2006·Journal of Bacteriology·Eliana De GregorioPier Paolo Di Nocera
Jul 14, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jianying GuYufeng Wang
Mar 31, 2010·Briefings in Functional Genomics·Magali Naville, Danie Gautheret
Sep 28, 1998·Journal of Bacteriology·P HuE Garcia
Mar 30, 2002·Journal of Clinical Microbiology·Xiao-Zhe HuangLuther E Lindler

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