PMID: 11341935May 9, 2001Paper

Molecular characterization of L-amino acid oxidase from Agkistrodon halys blomhoffii with special reference to platelet aggregation

Biochimica Et Biophysica Acta
H TakatsukaY Fujimura

Abstract

L-Amino acid oxidase (LAO, EC 1.4.3.2) is widely distributed in snake venom, and induces apoptosis in vascular endothelial cells, causing prolonged bleeding from vessel walls at bite sites. The effect of snake venom LAOs on platelet function is controversial. Further, we have little information on their structural characterization. We purified M (mamushi)-LAO, a single-chain glycoprotein with a molecular mass of 60 kDa and a pI of 4.9, from Agkistrodon halys blomhoffii (Japanese mamushi) venom, and determined the N-terminal and several internal amino acid sequences of this enzyme. Molecular cloning based on these data was conducted to elucidate its full-length cDNA structure (2192 nucleotides), which includes a putative 18 amino acid residue signal peptide and a 504 residue mature subunit. The predicted M-LAO translation product shares 87.3% identity with that of Crotalus adamanteus (Southeastern diamondback rattlesnake) LAO. M-LAO, up to a final concentration of 2.6 microM, inhibited both agonist- and shear stress-induced platelet aggregation (SIPA) dose-dependently. In agonist-induced platelet aggregation, M-LAO predominantly inhibited the second aggregation, but with a marginal inhibition of the first. In SIPA, the inhibitio...Continue Reading

References

May 1, 1976·British Journal of Haematology·R RodveinP H Levine
Jun 1, 1992·The International Journal of Biochemistry·N H Tan, S Swaminathan
Jan 1, 1991·Haemostasis·D PraticòF Violi
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Apr 1, 1972·The Tohoku Journal of Experimental Medicine·O HigashiK Konno
Feb 1, 1983·The Journal of Clinical Investigation·R R MontgomeryM Corcoran
Nov 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·Z Y LiE C Lian
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y UsamiK Titani
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian

❮ Previous
Next ❯

Citations

Oct 12, 2013·Fish & Shellfish Immunology·Ruijun LiAnxing Li
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Steven D Aird
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·Xiao-Yan Du, Kenneth J Clemetson
Apr 18, 2003·Protein Expression and Purification·Birgit Geueke, Werner Hummel
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Annette FaustDietmar Schomburg
Jul 11, 2014·Toxicon : Official Journal of the International Society on Toxinology·Zaineb Abdelkafi-KoubaaNaziha Marrakchi
Aug 12, 2014·Journal of Proteomics·Vincent L VialaPatrick J Spencer
Dec 1, 2009·Toxicon : Official Journal of the International Society on Toxinology·Alba Fabiola Costa TorresAlice M C Martins
Jun 16, 2009·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongQiu-Min Lu
Jun 2, 2009·Toxicon : Official Journal of the International Society on Toxinology·Marcelo L SantoroSolange M T Serrano
Sep 23, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Raquel Melo AlvesSuely Vilela Sampaio
May 27, 2008·Toxicon : Official Journal of the International Society on Toxinology·Ying JiaJohn C Pérez
Nov 7, 2007·Toxicon : Official Journal of the International Society on Toxinology·Marcus Davis Machado BragaHelena Serra Azul Monteiro
Nov 13, 2007·The Journal of Surgical Research·Danielle K MacarioGary B Nackman
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Q LuK J Clemetson
Mar 19, 2008·Basic & Clinical Pharmacology & Toxicology·Mariana M de Vieira SantosDenise Fecchio
Jun 9, 2004·Free Radical Biology & Medicine·Sumio YanoNoriko Yano
Sep 10, 2003·Biochemical and Biophysical Research Communications·Yu-Jie ZhangYun Zhang
Apr 8, 2011·The FEBS Journal·Tse Siang KangR Manjunatha Kini
Apr 17, 2014·BioMed Research International·Luiz Fernando M IzidoroAndreimar M Soares
May 5, 2011·Biochimica Et Biophysica Acta·Gustavo B NaumannEladio F Sanchez
Feb 26, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mari SamelEne Siigur
May 15, 2012·Toxicon : Official Journal of the International Society on Toxinology·Chunmei GuoMing-Zhong Sun
Jan 5, 2013·Toxicon : Official Journal of the International Society on Toxinology·Leidy Johana VargasJuan Calvete
Jun 19, 2016·Toxicon : Official Journal of the International Society on Toxinology·Tuila Leveghim TeixeiraMaurício Ventura Mazzi
Jul 11, 2016·International Journal of Biological Macromolecules·Patrícia H RibeiroAndreimar M Soares
Jul 11, 2006·Toxicon : Official Journal of the International Society on Toxinology·Külli TõnismägiEne Siigur
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Hong TaiYun Zhang
Jun 5, 2007·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYun Zhang
Nov 26, 2005·Toxicon : Official Journal of the International Society on Toxinology·Marcos H ToyamaMarasses C Fonteles
Sep 4, 2016·Acute Medicine & Surgery·Osamu OkamotoNobuhiro Inagaki
May 13, 2014·Journal of Biochemical and Molecular Toxicology·Ziad-Meziane Hanane-Fadila, Laraba-Djebari Fatima
Feb 19, 2013·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E V LukashevaT T Berezov
Jan 31, 2021·Toxicon : Official Journal of the International Society on Toxinology·Eduardo Oliveira Venancio de LimaKaren de Morais-Zani
Jun 14, 2018·Toxicon : Official Journal of the International Society on Toxinology·Walaa H SalamaMamdouh M Ali
May 29, 2002·Biochimica Et Biophysica Acta·Sangeeta S ChavanCharles C Chu
May 15, 2004·Bioorganic & Medicinal Chemistry·Rodrigo G StábeliAndreimar M Soares
Jul 1, 2006·Bioorganic & Medicinal Chemistry·Luiz Fernando M IzidoroVeridiana M Rodrigues
Feb 27, 2007·International Journal of Biological Macromolecules·Rodrigo G StábeliAndreimar M Soares

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.