Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells

International Journal of Oncology
A SalviG De Petro

Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. We have previously reported that LASP-1 is a downstream protein of the urokinase type plasminogen activator (uPA). Here we investigated the role of LASP-1 in HCC by a molecular and biological characterization of LASP-1 expression in human HCC specimens and in cultured HCC cells. We determined the LASP-1 mRNA expression levels in 55 HCC cases with different hepatic background disease. We identified 3 groups of patients with high, equal or low LASP-1 mRNA levels in HCC tissues compared to the peritumoral (PT) tissues. In particular we found that i) the HCCs displayed a higher LASP-1 mRNA level in HCC compared to PT tissues; ii) the expression levels of LASP-1 mRNA in female HCCs were significantly higher compared to male HCCs; iii) the cirrhotic HCCs displayed a higher LASP-1 mRNA. Further, the biological characterization of the ectopic LASP-1 overexpression in HCC cells, using MALDI-TOF mass spectrometer on the LASP-1 co-immunoprecipitated fractions, displayed vimentin as a novel putative partner of LASP-1. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female HCCs and cirrhotic HCCs; and that LAS...Continue Reading

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Citations

Mar 31, 2018·Journal of Cellular Physiology·Hongjuan YouRenxian Tang
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Vitalba RuggieriDomenica Mangieri
Apr 27, 2019·Journal of Cellular Biochemistry·Yimeng Zhang, Liya Zhang
Jan 6, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongchen ZhangWei Gong
Sep 3, 2020·Cancers·Augustus M C TilleyDayanidhi Raman
Oct 10, 2018·Frontiers in Oncology·Elke Butt, Dayanidhi Raman
Jul 17, 2020·Disease Markers·Xiaojing YangJie Fu

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Methods Mentioned

BETA
biopsy
surgical resection
Assay
PCR
transfection
immunoprecipitation
electrophoresis
Fluorescence
co-immunoprecipitation
biopsies

Software Mentioned

kyplot
Aldente

Related Concepts

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