Molecular Characterization of Markers Associated With Antimicrobial Resistance in Neisseria gonorrhoeae Identified From Residual Clinical Samples

Sexually Transmitted Diseases
Johan H MelendezCharlotte A Gaydos

Abstract

The emergence and spread of antimicrobial-resistant (AMR) Neisseria gonorrhoeae (NG) is a major public health concern. In the era of nucleic acid amplifications tests, rapid and accurate molecular approaches are needed to help increase surveillance, guide antimicrobial stewardship, and prevent outbreaks. Residual urethral swabs, collected prospectively in the Baltimore City Health Department during a 6-month period, were analyzed by real-time polymerase chain reaction assays for NG DNA and AMR determinants to fluoroquinolones, penicillin, and extended-spectrum cephalosporins. N. gonorrhoeae DNA was detected in 34.8% (73/210) of samples, including 67.3% (68/101) of the swabs that had been previously identified as NG positive by culture. Markers associated with decreased susceptibility to fluoroquinolones were detected in 22.4% of the polymerase chain reaction NG-positive samples. The rate of penicillinase-producing NG was very low (1.6%), and no markers associated with decreased susceptibility to extended-spectrum cephalosporins were detected in this cohort of men using the AMR assays herein described. Detection of molecular markers associated with AMR in NG can be performed directly from residual clinical samples, although the ...Continue Reading

References

Feb 25, 2005·Sexually Transmitted Diseases·Sepehr N TabriziSuzanne M Garland
Feb 3, 2007·Journal of Clinical Microbiology·Mark J SiednerJeffrey D Klausner
Jan 14, 2012·Annals of the New York Academy of Sciences·Magnus Unemo, William M Shafer
Feb 10, 2012·The New England Journal of Medicine·Gail A BolanJudith N Wasserheit
Nov 4, 2015·JAMA : the Journal of the American Medical Association·Robert D KirkcaldyJohn R Papp
Nov 6, 2015·The Journal of Antimicrobial Chemotherapy·Cameron BuckleyUNKNOWN GRAND study investigators
Jul 16, 2016·MMWR. Surveillance Summaries : Morbidity and Mortality Weekly Report. Surveillance Summaries·Robert D KirkcaldyElizabeth Torrone
Jan 11, 2017·Nature Reviews. Urology·Magnus Unemo, Jorgen S Jensen
Aug 19, 2017·Emerging Infectious Diseases·Ashima A BhattiJeffrey D Klausner

❮ Previous
Next ❯

Citations

Dec 20, 2019·Journal of Clinical Microbiology·Teresa L StreetDavid W Eyre
May 14, 2021·Science Translational Medicine·Alexander Y TrickTza-Huei Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.