Molecular Characterization of Multidrug-Resistant Pseudomonas aeruginosa Isolates in Hospitals in Myanmar

Antimicrobial Agents and Chemotherapy
Tatsuya TadaHtay Htay Tin

Abstract

The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR P. aeruginosa strains isolated from hospitals in Myanmar. Forty-five MDR P. aeruginosa isolates obtained from different patients in seven hospitals in Myanmar were screened using the broth microdilution method. The whole genomes of the MDR isolates were sequenced using a MiSeq platform (Illumina). Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence types were deduced, and drug resistance genes were identified. Of the 45 isolates, 38 harbored genes encoding carbapenemases, including DIM-1, IMP-1, NDM-1, VIM-2, and VIM-5, and 9 isolates had genes encoding 16S rRNA methylases, including RmtB, RmtD3, RmtE, and RmtF2. Most MDR P. aeruginosa strains isolated in Myanmar belonged to sequence type 1047 (ST1047). This is the first molecular epidemiological analysis of MDR P. aeruginosa clinical isolates in Myanmar. These findings strongly suggest that P. aeruginosa ST1047 strains harboring carbapenemases, including DIM-, IMP-, NDM-, and VIM-type metallo-β-lactamases, have been spreading thr...Continue Reading

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Citations

Dec 18, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jun Sung HongSeok Hoon Jeong
Jan 15, 2021·BMJ Open Respiratory Research·Iwein GyselinckRobin Vos
Feb 19, 2021·Pharmacological Reviews·Mariana Angoa-Pérez, Donald M Kuhn
Mar 9, 2021·Frontiers in Microbiology·Eun-Jeong Yoon, Seok Hoon Jeong
Oct 5, 2020·Infectious Disease Clinics of North America·Jun-Ichi WachinoYoshichika Arakawa
Jul 21, 2021·Journal of Global Antimicrobial Resistance·Toshihiro TakahashiJeevan B Sherchand

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