Abstract
The objective of this study was to investigate the occurrence of multidrug-resistant Escherichia coli in cows with clinical mastitis in 42 different dairy farms located in the Bordj Bou Arreridj region of Algeria. Milk samples were cultured on Columbia blood agar, and isolates were then identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. In total, 200 samples were screened and 52 E. coli strains confirmed as causative agents were obtained. The antimicrobial susceptibility testing was performed by disk diffusion method. Antibiotic resistance genes, including those conferring resistance to extended-spectrum β-lactamases (i.e., blaTEM, blaSHV, and blaCTX-M), tetracyclines (tetA, tetB, tetC, and tetJ), aminoglycosides [aph(3'), aac(3'), aac(6'), ant, aad, and armA], and quinolones (qnrA and qnrB) were amplified by standard PCR and sequenced when positive. Transferability of resistance genes has been investigated by conjugation experiments and multilocus sequence typing. The most frequently observed resistance was to amoxicillin (86.5%), followed by tetracycline (75%), amoxicillin-clavulanic acid (59.6%), trimethoprim-sulfamethoxazole (36.5%), doxycycline (13.5%), and ciprofloxacin (13.5%). Mu...Continue Reading
References
Feb 23, 2011·Microbiology and Immunology·Ashraf M Ahmed, Tadashi Shimamoto
Jul 24, 2012·Journal of Dairy Science·V SainiH W Barkema
Nov 7, 2012·Veterinary Microbiology·Safia DahmenMarisa Haenni
Sep 18, 2013·Veterinary Microbiology·D Jones-DiasM Caniça
Nov 20, 2013·Antimicrobial Agents and Chemotherapy·Dorina TimofteNicola J Williams
May 21, 2014·PloS One·Kyung-Hwan OhSeung-Hak Cho
May 25, 2015·International Journal of Antimicrobial Agents·Valérie ThomasMichel Vallé
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
May 2, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Massilia YousfiChristophe De Champs
Jul 16, 2016·BMC Veterinary Research·Juliana Fortes Vilarinho BragaCatherine Schouler
Aug 28, 2016·Veterinary Microbiology·Christin FreitagStefan Schwarz
Mar 21, 2017·Journal of Dairy Science·Dong-Seob TarkSuk-Kyung Lim
May 10, 2017·BMC Genomics·Andreas LeimbachUlrich Dobrindt
Aug 22, 2017·Tropical Animal Health and Production·Eugene MazimpakaOkwee-Acai James
Sep 28, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·David EisenbergerGiuseppe Valenza
Nov 21, 2017·Journal of Dairy Science·Pamela L Ruegg
Nov 23, 2017·Oncotarget·Tariq AliBo Han
Jan 3, 2018·Veterinary Microbiology·Anno de JongUNKNOWN VetPath Study Group
Feb 6, 2018·Journal of Dairy Science·Shiyao ZhangBo Han
Apr 2, 2018·Journal of Dairy Science·Feng YangXurong Wang
Oct 9, 2018·Animal Science Journal = Nihon Chikusan Gakkaihō·Hossein JamaliMohammed Aïder
Sep 27, 2019·Frontiers in Microbiology·Pei ZhangZengqi Yang
Nov 23, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Gang LiuBo Han
Nov 26, 2019·Saudi Journal of Biological Sciences·Fuad AmeenAbdullah A Alarfaj