Molecular Characterization of Multidrug-Resistant Escherichia coli Isolated from Milk of Dairy Cows with Clinical Mastitis in Algeria.

Journal of Food Protection
Sedrati TaharSeydina M Diene

Abstract

The objective of this study was to investigate the occurrence of multidrug-resistant Escherichia coli in cows with clinical mastitis in 42 different dairy farms located in the Bordj Bou Arreridj region of Algeria. Milk samples were cultured on Columbia blood agar, and isolates were then identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. In total, 200 samples were screened and 52 E. coli strains confirmed as causative agents were obtained. The antimicrobial susceptibility testing was performed by disk diffusion method. Antibiotic resistance genes, including those conferring resistance to extended-spectrum β-lactamases (i.e., blaTEM, blaSHV, and blaCTX-M), tetracyclines (tetA, tetB, tetC, and tetJ), aminoglycosides [aph(3'), aac(3'), aac(6'), ant, aad, and armA], and quinolones (qnrA and qnrB) were amplified by standard PCR and sequenced when positive. Transferability of resistance genes has been investigated by conjugation experiments and multilocus sequence typing. The most frequently observed resistance was to amoxicillin (86.5%), followed by tetracycline (75%), amoxicillin-clavulanic acid (59.6%), trimethoprim-sulfamethoxazole (36.5%), doxycycline (13.5%), and ciprofloxacin (13.5%). Mu...Continue Reading

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Jul 24, 2012·Journal of Dairy Science·V SainiH W Barkema
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
May 2, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Massilia YousfiChristophe De Champs
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Aug 22, 2017·Tropical Animal Health and Production·Eugene MazimpakaOkwee-Acai James
Sep 28, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·David EisenbergerGiuseppe Valenza
Nov 21, 2017·Journal of Dairy Science·Pamela L Ruegg

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Citations

Apr 30, 2021·Frontiers in Microbiology·Linda FalgenhauerEugen Domann

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