PMID: 9178268May 1, 1997Paper

Molecular characterization of myosin IB from the lower eukaryote Entamoeba histolytica, a human parasite

Molecular and Biochemical Parasitology
M VargasNancy Guillén

Abstract

The complete amino acid sequence of the Entamoeba histolytica unconventional myosin IB (Eh-myosin IB) is reported. Sequencing of overlapping cDNA fragments reveals a single open reading frame which predicts a 130 kDa protein of 1049 aa. Eh-myosin IB presents the three characteristics domains of myosins I subclass 1. This protein presents homology with myosins IB from other amoebae, but striking homologies with vertebrate unconventional myosins were also observed. The predicted actin and ATP-binding sites are located in the head domain. The heavy chain phosphorylation region is homologous to metazoan myosins I with an acidic residue present at the phosphorylation site. In the neck domain, an IQ motif indicates potential binding of calmodulin to the myosin I heavy chain. In the tail of Eh-myosin IB the three characteristic regions of myosin I are found. A putative membrane binding domain a very short domain rich in alanine and proline we demonstrate to be functional for actin binding, and the src-homology 3 domain. The Entamoeba histolytica myosin IB is the first unconventional myosin so far described in a lower eukaryote.

Citations

May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·K H KirschH Hanafusa
Oct 16, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S Arias-NegreteF Murillo-Jasso
Sep 18, 2007·Experimental Parasitology·Bibiana Chávez-MunguíaAdolfo Martínez-Palomo
Dec 29, 2005·Archives of Medical Research·Elisabeth Labruyère, Nancy Guillén
Sep 25, 2010·Cellular & Molecular Biology Letters·Barbara Addario, Lars Backman
Apr 8, 2015·International Journal for Parasitology·C M GuptaA A Sahasrabuddhe
May 5, 2009·Progress in Histochemistry and Cytochemistry·Wanderley de SouzaNarcisa L Cunha-e-Silva
Sep 7, 2000·The Journal of Cell Biology·E M Neuhaus, T Soldati
Dec 29, 2005·Archives of Medical Research·Isaura MezaMiguel A Vargas
Aug 5, 2017·Acta Crystallographica. Section D, Structural Biology·Gunjan GautamSamudrala Gourinath

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