PMID: 9536440Apr 16, 1998Paper

Molecular characterization of myosin V from Drosophila melanogaster

Journal of Muscle Research and Cell Motility
N Bonafé, J R Sellers

Abstract

Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement. In this paper we report the molecular characterization of the Drosophila myosin V, identified by reverse genetics. The gene encodes a 1792-residue, 207 kDa heavy chain polypeptide which possesses a typical head or motor domain of 771 residues, a region of six IQ motifs (139 residues) which serve as potential calmodulin/light chain binding sites at the head/tail junction, and a tail domain of 882 residues containing sequences of putative alpha-helical coiled-coils required for dimerization of the molecule and sequences of non-helical structure at the C-terminal end. Based on Southern blot analyses and chromosomal localization, evidence is presented for a single Drosophila myosin V gene. RNA analyses revealed a doublet of transcripts of about 6 kb, expressed throughout the lifetime of a fly but particularly abundant in the early stages of embryonic development (maternally contributed), in the ectodermic tissue of the hindgut starting at stage 16, and in the adult head. These results suggest that myosin V may be involved in processes required in a variety of cell types in Drosophila. We have also mapp...Continue Reading

Citations

Jun 16, 2001·Molecular Biology of the Cell·L A LapierreJ R Goldenring
Jun 26, 2002·Molecular Biology and Evolution·George TzolovskyMary Bownes
Feb 8, 2000·The Journal of Biological Chemistry·F WangJ R Sellers
Jun 28, 2005·The Journal of Biological Chemistry·Judit TóthJames R Sellers
Feb 25, 2021·Molecular Biology of the Cell·Antonia WiegeringChristoph Gerhardt
Aug 30, 2005·Developmental Biology·Valerie MermallMark S Mooseker
Aug 15, 2006·Biochimica Et Biophysica Acta·Christopher B O'ConnellMark S Mooseker
Jun 2, 2009·Current Biology : CB·Jana KraussAnne Ephrussi

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