Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya

International Journal of Microbiology
Irene N OgaliSheila C Ommeh

Abstract

Newcastle disease (ND) is a serious disease of poultry that causes significant economic losses. Despite rampant ND outbreaks that occur annually in Kenya, the information about the NDV circulating in Kenya is still scarce. We report the first countrywide study of NDV in Kenya. Our study is aimed at evaluating the genetic characteristics of Newcastle disease viruses obtained from backyard poultry in farms and live bird markets in different regions of Kenya. We sequenced and analyzed fusion (F) protein gene, including the cleavage site, of the obtained viruses. We aligned and compared study sequences with representative NDV of different genotypes from GenBank. The fusion protein cleavage site of all the study sequences had the motif 112RRQKRFV118 indicating their velogenic nature. Phylogenetic analysis revealed that the NDV from various sites in Kenya was highly similar genetically and that it clustered together with NDV of genotype V. The study samples were 96% similar to previous Ugandan and Kenyan viruses grouped in subgenotype Vd This study points to possible circulation of NDV of similar genetic characteristics between backyard poultry farms and live bird markets in Kenya. The study also suggests the possible spread of velog...Continue Reading

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Citations

Jul 5, 2019·Microbiology Resource Announcements·Iryna V GoraichukDavid L Suarez
Sep 11, 2019·Tropical Animal Health and Production·Auleria A ApopoClaudio L Afonso
Nov 23, 2021·Frontiers in Veterinary Science·Vinay G JoshiSagar M Goyal

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Datasets Mentioned

BETA
KY007043
KY007063
MG988405
KY007050

Methods Mentioned

BETA
electrophoresis
PCR
PCRs
reverse transcription PCR
fusion

Software Mentioned

MUSCLE
Chromas Lite
DnaSP
MEGA ( Molecular Evolutionary Genetics Analysis )

Related Concepts