Molecular Characterization of Norovirus Strains Isolated from Older Children and Adults in Impoverished Communities of Vhembe District, South Africa

Advances in Virology
G MulondoNatasha Potgieter

Abstract

Human norovirus (NoV) is an etiological agent associated with acute gastroenteritis (AGE) in both children and adults worldwide. However, very few studies have been reported on the prevalence and genetic diversity of NoV strains in children older than 5 years of age and adults with little or inadequate water and sanitation conditions. The aim of this study was assessing the prevalence of the human norovirus in older children and adults suffering with diarrhoea from rural communities in the Vhembe district, Limpopo province. Between August 2017 and October 2018, stool samples were collected from outpatients suffering from AGE and screened for NoV strains using the RIDA©GENE norovirus I and II real-time one-step RT-PCR. RNA extracts of NoV-positive samples were subjected to RT-PCR amplification and nucleotide sequencing to genotype the positive NoV strains. Out of 80 collected stool samples, 13 (16%) were tested positive for norovirus. Genogroup GII was identified in 6/13 (46%) samples and genogroup GI in 7/13 (54%) samples. The sequence analyses showed multiple genotypes including GII.Pg, GII.1, GII.2, GII.4, and GI.3. Phylogenetic analysis revealed the relatedness of NoV genotypes identified with other strains reported globally...Continue Reading

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Jul 1, 1993·Virology·X JiangM K Estes
Sep 23, 1997·Scandinavian Journal of Gastroenterology·S J Lewis, K W Heaton
Dec 14, 2005·Virology·Du-Ping ZhengStephan S Monroe
Feb 6, 2009·Journal of Clinical Microbiology·Aziza H KamelGaël Belliot
Jan 22, 2011·Epidemiology and Infection·M Riera-MontesM Löfdahl
Apr 26, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A KronemanM Koopmans
Jul 25, 2012·Microbiology and Immunology·Sanghun ParkHyunsoo Kim
Feb 16, 2013·Food and Environmental Virology·Elisabeth MathijsEtienne Thiry
Apr 26, 2013·Archives of Virology·Annelies KronemanMarion Koopmans
Jul 16, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Dieter HoffmannUlrike Protzer
Sep 7, 2013·Journal of Medical Virology·Chi-Neu TsaiShih-Yen Chen
Oct 15, 2013·Journal of Medical Virology·Janet MansMaureen B Taylor
Nov 16, 2013·Current Opinion in Gastroenterology·Sasirekha RamaniMary K Estes
Feb 8, 2014·PloS One·Shaowei SangYunxi Liu
Apr 29, 2014·Diagnostic Microbiology and Infectious Disease·N L DunbarJ A Marshall
Jun 25, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sabrina MoyoNina Langeland
Jul 2, 2014·The Lancet Infectious Diseases·Sharia M AhmedBenjamin A Lopman
Jul 6, 2014·Journal of Clinical Microbiology·Jan Vinjé
Jan 9, 2015·Epidemiology and Infection·P LouryB Hubert
Oct 6, 2015·Tropical Medicine & International Health : TM & IH·Jean Pierre KabueNatasha Potgieter
Feb 11, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Juliana das Merces HernandezYvone Benchimol Gabbay
Mar 8, 2016·Journal of Medical Virology·Leesa D BrugginkJohn A Marshall
Apr 27, 2016·PloS One·Janet MansMaureen B Taylor
Oct 25, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jean Pierre KabueNatasha Potgieter
Feb 6, 2017·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Victor S SantosLuis E Cuevas
May 4, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kanittapon SupadejNiwat Maneekarn
Aug 8, 2017·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jean Pierre KabueNatasha Potgieter
Aug 28, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Takako UtsumiIkuo Shoji
Sep 16, 2017·Infectious Disease Clinics of North America·Cristina V CardemilAron J Hall
Mar 30, 2018·Emerging Microbes & Infections·Jennifer H LunPeter A White
Apr 28, 2018·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·N KanwarR Selvarangan
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jul 7, 2018·Journal of Medical Virology·Nikolaos SiafakasSpyridon Pournaras
Aug 11, 2018·Expert Review of Vaccines·Claire P MattisonAron J Hall
Dec 16, 2018·Environment International·Tulio M FumianMarize P Miagostovich

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
MK623268
MK671479
MN156316
MN473875
MN473876

Methods Mentioned

BETA
PCR

Software Mentioned

Finch TV
MEGA
BLAST
Noronet
Paleontological Statistics ( PAST )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.