Molecular characterization of occult hepatitis B virus in genotype E-infected subjects

The Journal of General Virology
Astrid ZahnJean-Pierre Allain

Abstract

Occult hepatitis B virus (HBV) infection (OBI), defined as the presence of HBV DNA without detectable HBV surface antigen (HBsAg), is frequent in west Africa, where genotype E is prevalent. The prevalence of OBI in 804 blood donors and 1368 pregnant women was 1.7 and 1.5%, respectively. Nine of 32 OBI carriers were evaluated with HBV serology, viral load and complete HBV genome sequence of two to five clones. All samples except one were anti-HBV core antigen-positive and three contained antibodies against HBsAg (anti-HBs). All strains were of genotype E and formed quasispecies with 0.20-1.28% intra-sample sequence variation. Few uncommon mutations (absent in 23 genotype E reference sequences) were found across the entire genome. Two mutations in the core region encoded truncated or abnormal capsid protein, potentially affecting viral production, but were probably rescued by non-mutated variants, as found in one clone. No evidence of escape mutants was found in anti-HBs-carrying samples, as the 'a' region was consistently wild type. OBI carriers constitute approximately 10% of all HBV DNA-viraemic adult Ghanaians. OBI carriers appear as a disparate group, with a very low viral load in common, but multiple origins reflecting deca...Continue Reading

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Citations

Sep 7, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Xianlin YeChengyao Li
Mar 18, 2011·Journal of Clinical Microbiology·Xin ZhengChengyao Li
Feb 7, 2012·Biologicals : Journal of the International Association of Biological Standardization·Jean-Pierre AllainUNKNOWN ISBT HBV Safety Collaborative Group
Mar 26, 2009·Journal of Medical Virology·Antigoni KatsoulidouAngelos Hatzakis
May 13, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Pierre AllainDaniel Candotti
Jun 17, 2011·Journal of Medical Virology·Laura E CoxJean-Pierre Allain
Feb 1, 2013·Transfusion·Jean-Pierre AllainMichael Chudy
Apr 20, 2011·Journal of Medical Virology·Penelope GarmiriJean-Pierre Allain
Jul 22, 2010·Journal of Medical Virology·Sonia RomanArturo Panduro
May 23, 2014·Transfusion·Ohene Opare-SemShirley Owusu-Ofori
Jan 15, 2010·Vox Sanguinis·C NiederhauserM Stolz
Mar 27, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Cecilia M DelfinoMirna M Biglione
Jul 23, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Joseph C ForbiYury E Khudyakov
Apr 5, 2017·Tropical Medicine & International Health : TM & IH·Jennifer GrantClaudia Hawkins
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Dec 27, 2019·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jiawen WangTingting Li

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