Molecular characterization of protein p50 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

Journal of Basic Microbiology
Liang JinXiulian Sun

Abstract

Genome segment 7 of the 10-segmented RNA genomes of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) comprises 1502 nucleotides with one ORF of 1347 bp. This ORF was predicted to encode a protein of 448 amino acids with a molecular mass of 49,756 Da (p50). Antisera against both p50 and an antigen domain (AD) near the N-terminus of p50 specifically bound to a viral structural protein of ca. 33 kDa (V5), indicating that V5 was an N-terminal product of p50. Immunoblotting analysis with anti-p50 antibodies detected p50 and V5 molecules in the host midguts three days and five days post infection, respectively. The intracellular localization of p50 protein was examined by expressing truncations of p50 fused with GFP in recombinant baculovirus-infected Sf9 cells. The p50 protein was present in the cytoplasm of the cells, and the N-terminal portion (67-135 aa) of the protein played a key role in this localization.

References

Mar 1, 1992·Journal of Virology·P R LambdenI N Clarke
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E Johnson
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·G BarbaC Bréchot
Oct 29, 2000·Advances in Virus Research·J A LawtonB V Prasad
Mar 8, 2005·Journal of Thrombosis and Haemostasis : JTH·M H A BosK Mertens
Jun 9, 2005·The Journal of Prosthetic Dentistry·Rifat GozneliZerrin Fidan Akalin
Apr 28, 2007·Journal of Invertebrate Pathology·Yasuhisa Kunimi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.