Molecular characterization of RhD variant phenotypes among blood donors: A study from the coastal region of India.

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
Isha PolavarapuSwati Kulkarni

Abstract

RhD expression varies with population and ethnicity. Accurate typing of RhD antigen among blood donors is important to prevent development of anti-D among recipients of blood transfusion. We aimed to screen blood donors for variant D phenotypes and accurately characterize them by genotyping. We have done prospective study on blood donors by performing RhD typing using three different commercial monoclonal anti-D reagents by both column agglutination and conventional tube techniques. Samples that showed ambiguous results were further screened with the Bio-Rad Partial RhD typing kit. Minor phenotyping for C, c, E, e antigens was performed. Multiplex PCR and Sequencing of all RHD exons with Sanger's sequencing was performed for molecular characterization of variant D. A total of 16,974 blood donors were screened during the study period. Among them, 31 (0.18 %) donors were found to have a RhD variant phenotype. The male to female ratio was 10:1. The presence of 'C' antigen was noted among all RhD variant samples. Serological typing identified two samples as DV phenotype and the rest could not be characterized. Molecular genotyping characterized 90.3 % of the samples as Indian specific weak D type 150 variants. Three samples were su...Continue Reading

References

Apr 11, 2008·Transfusion Medicine·S KulkarniK Ghosh
Aug 19, 2010·American Journal of Clinical Pathology·Dehua WangKaren Quillen
Sep 23, 2010·Asian Journal of Transfusion Science·R N MakrooN L Rosamma
Feb 26, 2013·British Journal of Haematology·Geoff Daniels
Jun 27, 2018·Journal of Clinical Laboratory Analysis·Marcia Regina DezanCarla L Dinardo
May 16, 2019·Hematology, Transfusion and Cell Therapy·Rajeswari Subramaniyan
Jun 14, 2019·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Gordana GuzijanMarko Lilić
Feb 29, 2020·Acta Clinica Belgica·Sreelekshmi SPoornima Baliga B
Jun 5, 2020·Transfusion Medicine·Garima MishraSwati Kulkarni

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