Molecular Characterization of Rhynchosia mosaic virus-Puerto Rico Associated with Symptomatic Rhynchosia minima and Cajanus cajan in Puerto Rico

Plant Disease
A M IdrisJ K Brown

Abstract

A begomovirus (family Geminiviridae) has long been suspected to be associated with Rhynchosia mosaic (RhM) disease of Rhynchosia minima (L.) DC., a weed that is widespread in Puerto Rico (PR). The suspect virus has been transmitted by the Sida biotype of Bemisia tabaci (Genn.) and has been designated RhM virus-PR (RhMV-PR) (1) (synonym, Rhynchosia mosaic virus [RMV]). RhM symptoms in R. minima included yellow foliar mosaic and stunting. The virus has a broad experimental host range and infects species in the Fabaceae, including R. minima, pigeon pea (Cajanus cajan (L.) Millsp.), and Clitoria falcata L. (1). However, until now RhMV has not been identified from naturally infected pigeon pea or Clitoria falcata. R. minima and C. cajan plants exhibiting yellow foliar mosaic and stunting symptoms were collected in Puerto Rico. Using the B biotype of B. tabaci as the vector, their whitefly transmissibility from the respective source plant to R. minima and C. cajan test plants was confirmed, and symptoms in inoculated host were indistinguishable for both isolates. Using polymerase chain reaction (PCR) and primers (2), three amplicons were obtained and cloned for each isolate. PCR products (1.1 and 2.1 kbp) were assembled (~200 nucleot...Continue Reading

Citations

Sep 21, 2010·Archives of Virology·Elvira Fiallo-OlivéYamila Martínez-Zubiaur

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