Molecular characterization of selected multidrug resistant Pseudomonas from water distribution systems in southwestern Nigeria

Annals of Clinical Microbiology and Antimicrobials
Ayodele T AdesojiIsaac O Olatoye

Abstract

Persistence of antibiotic resistant bacteria, including multidrug resistant (MDR) pseudomonads, is an important environmental health problem associated with drinking water distribution systems (DWDS) worldwide. There is paucity of data on the molecular characteristics of antibiotic resistance genes and their mode of transfer among pseudomonads from DWDS located in resource-challenged areas such as southwestern Nigeria. MDR pseudomonads (n = 22) were selected from a panel of 296 different strains that were isolated from treated and untreated water in six DWDS located across southwest Nigeria. Primarily, the isolated pseudomonads strains were identified by 16S rDNA sequencing and antibiotic-resistance testing was completed using agar breakpoints assays. The final panel of strains of resistant to more than three classes of antibiotics (i.e. MDR), were further characterized by PCR genotyping, Sanger sequencing, and plasmid profiling. Pseudomonad resistance to gentamicin and streptomycin ranged from 22.7 to 54.6 % while resistance to tetracycline, ceftiofur and sulphamethoxazole ranged from 40.9 to 77.3 %. The most commonly detected antibiotic resistance genes were tet(A) (31.8 % of isolates), sul1 (31.8 %), bla TEM (40.9 %) and aph...Continue Reading

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Citations

Mar 31, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christy Manyi-LohAnthony Okoh
Oct 27, 2016·BMC Complementary and Alternative Medicine·Moses AbialaSunday Akinyemi
Dec 8, 2020·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Héctor SambranoMagaly de Chial
Aug 29, 2021·Journal of Global Antimicrobial Resistance· SamreenHussein H Abulreesh

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Methods Mentioned

BETA
PCR
genotyping
electrophoresis

Software Mentioned

BLAST
CLUSTAL W
MEGA

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