Molecular characterization of Streptococcus agalactiae and Streptococcus uberis isolates from bovine milk.

Tropical Animal Health and Production
Bibek Ranjan ShomeHabibar Rahman

Abstract

Streptococci are one among the major mastitis pathogens which have a considerable impact on cow health, milk quality, and productivity. The aim of the present study was to investigate the occurrence and virulence characteristics of streptococci from bovine milk and to assess the molecular epidemiology and population structure of the Indian isolates using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Out of a total of 209 bovine composite milk samples screened from four herds (A-D), 30 Streptococcus spp. were isolated from 29 milk samples. Among the 30 isolates, species-specific PCR and partial 16S rRNA gene sequence analysis identified 17 Streptococcus agalactiae arising from herd A and 13 Streptococcus uberis comprising of 5, 7, and 1 isolates from herds B, C, and D respectively. PCR based screening for virulence genes revealed the presence of the cfb and the pavA genes in 17 and 1 S. agalactiae isolates, respectively. Similarly, in S. uberis isolates, cfu gene was present in six isolates from herd C, the pau A/skc gene in all the isolates from herds B, C, and D, whereas the sua gene was present in four isolates from herd B and the only isolate from herd D. On MLST analysis, all the S. agalacti...Continue Reading

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Citations

Jul 23, 2013·Tropical Animal Health and Production·Shahzad AliSyed M Jamal
Oct 29, 2015·Revista Argentina de microbiología·Melina S PerrigMaría Sol Barbagelata
Dec 22, 2015·Journal of Dairy Science·Kansuda LeelahapongsathonWitaya Suriyasathaporn
Apr 12, 2016·Journal of Dairy Science·J B W J CornelissenUNKNOWN 1Health4Food—Dutch Mastitis Diagnostics Consortium
Apr 12, 2015·Revista Argentina de microbiología·Elina B ReinosoLiliana M Odierno
Aug 6, 2019·Frontiers in Veterinary Science·Nadine KäppeliRoger Stephan
Aug 17, 2019·Frontiers in Bioengineering and Biotechnology·Sofia A M MartinsPaulo P Freitas
Jun 5, 2021·Folia Microbiologica·Aluminé S Fessia, Liliana M Odierno
Jul 27, 2021·Journal of Applied Microbiology·Jifeng ZengWeixin Zeng

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