PMID: 9662705Jul 15, 1998Paper

Molecular characterization of susceptible and naturally resistant strains of Trypanosoma cruzi to benznidazole and nifurtimox

Molecular and Biochemical Parasitology
Silvane M F MurtaAlvaro J Romanha

Abstract

Twenty-seven Trypanosoma cruzi strains, susceptible or naturally resistant to the nitroderivatives benznidazole and nifurtimox, were analyzed using the following molecular markers: (i) isoenzyme patterns of six enzymes; (ii) genetic variability assayed by randomly amplified polymorphic DNA (RAPD) with two different primers; and (iii) gene probes for P-glycoprotein (TcPGP), hypoxanthine-guanine phosphoribosyltransferase (HGPRT), the ribosomal RNA gene (rDNA) and the mini-exon gene (MEX), RAPD and isoenzyme profiles divided the T. cruzi strains into three groups, whereas the gene probes divided the T. cruzi strains in two groups. Strains classified as group I or II by RAPD or zymodemes Z1 or Z2 by isoenzyme analysis were either susceptible or naturally resistant to the nitroderivatives. In contrast, strains classified as group III by RAPD and zymodeme ZB by isoenzyme analysis were only drug susceptible and showed polymorphisms for HGPRT and TcPGP. No correlation was observed between drug susceptibility and polymorphisms of rDNA and MEX. Eighteen T. cruzi strains isolated from different geographic regions were included in this study. Thus, from a total of 45 T. cruzi strains analyzed, all 19 of zymodeme B were susceptible to the e...Continue Reading

References

Oct 1, 1992·Molecular and Biochemical Parasitology·A M MacedoS D Pena
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·A O LuquettiI Rodrigues
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S Filardi, Z Brener
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C MorelL Simpson
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·B Ullman
Jul 1, 1993·Molecular and Biochemical Parasitology·M SteindelA J Simpson
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TibayrencF J Ayala
Mar 1, 1993·The Journal of Eukaryotic Microbiology·M F DirieP R Gardiner
Jan 1, 1996·Molecular and Biochemical Parasitology·B DallagiovannaS Castanys
Jan 1, 1996·Revista Da Sociedade Brasileira De Medicina Tropical·S G Andrade, J B Magalhães
Dec 20, 1996·Molecular and Biochemical Parasitology·R P SoutoB Zingales
Mar 3, 1962·Nature·P H SNEATH, R R SOKAL

❮ Previous
Next ❯

Citations

Jul 7, 2011·Journal of Bioenergetics and Biomembranes·Thiago M SilvaFernanda R Gadelha
Sep 19, 2003·International Journal of Antimicrobial Agents·Dominique KerboeufGeorg von Samson-Himmelstjerna
Aug 11, 2000·Bioorganic & Medicinal Chemistry Letters·W HeV Chu
Apr 18, 2002·Bioorganic & Medicinal Chemistry Letters·Wei HeValeria Chu
Mar 4, 2000·Bioorganic & Medicinal Chemistry Letters·Y GongJ Gao
Dec 22, 1999·Trends in Microbiology·J C Manson
Sep 16, 2010·Expert Reviews in Molecular Medicine·Caroline JunqueiraRicardo T Gazzinelli
Oct 26, 2011·Journal of Medicinal Chemistry·Maria V PapadopoulouEls Torreele
May 4, 2012·Journal of Medicinal Chemistry·Maria V PapadopoulouJean-Robert Ioset
Mar 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shane R WilkinsonIan Cheeseman
Nov 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Caroline JunqueiraRicardo T Gazzinelli
Aug 16, 2013·The Journal of Infectious Diseases·Juan M BustamanteRick L Tarleton
May 5, 2010·Antimicrobial Agents and Chemotherapy·Antoaneta Y SokolovaAlan H Fairlamb
Aug 4, 2010·Antimicrobial Agents and Chemotherapy·Chris BotShane R Wilkinson
Dec 6, 2006·Antimicrobial Agents and Chemotherapy·S Radhika PrathalinghamJohn M Kelly
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Max Jean de Ornelas ToledoMarta de Lana
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Mario FaundezJuan Diego Maya
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Diana VillarrealMichel Tibayrenc
Oct 4, 2006·Biological & Pharmaceutical Bulletin·Patrícia Shima LuizeCelso Vataru Nakamura
Jan 18, 2014·Parasitology Research·Cristina Fonseca-BerzalAlicia Gómez-Barrio
Oct 21, 2006·Parasitology Research·Juliana SaraivaSérgio de Albuquerque
Jul 15, 2015·Expert Review of Anti-infective Therapy·Louisa A MessengerCaryn Bern
Jan 10, 2016·Acta Tropica·José BermudezSantiago Palma
Sep 24, 2008·Expert Opinion on Investigational Drugs·Charles MadyJosé Leão de Souza
Jan 10, 2012·Molecular and Biochemical Parasitology·Juliana M FigueiredoNorton Heise
May 4, 2010·Experimental Parasitology·Aurea Regina Telles PupulinCiomar Aparecida Bersani-Amado
Nov 28, 2009·Advanced Drug Delivery Reviews·Eder Lilia Romero, Maria Jose Morilla
Jan 9, 2010·Journal of Proteomics·Patricia CuervoJose B De Jesus
Jan 10, 2009·Experimental Parasitology·Daniel B LiarteAlvaro J Romanha
Mar 31, 2007·International Journal of Antimicrobial Agents·Juan M BustamantePatricia Paglini-Oliva
Aug 2, 2015·The Lancet Infectious Diseases·Jose A Pérez-MolinaRogelio López-Vélez
May 26, 2009·Molecular and Biochemical Parasitology·Virginie SauvageIsabelle Villena

❮ Previous
Next ❯

Related Concepts