PMID: 2120195Oct 1, 1990Paper

Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli

Journal of Bacteriology
M B TobinJ R Miller

Abstract

The final step in the biosynthesis of beta-lactam antibiotics in Penicillium chrysogenum and Aspergillus nidulans involves removal of the L-alpha-aminoadipyl side chain from isopenicillin N (IPN) and exchange with a nonpolar side chain. The enzyme catalyzing this reaction, acyl-coenzyme A:isopenicillin N acyltransferase (acyltransferase), was purified from P. chrysogenum and A. nidulans. Based on NH2-terminal amino acid sequence information, the acyltransferase gene (penDE) from P. chrysogenum and A. nidulans were cloned. In both organisms, penDE was located immediately downstream from the isopenicillin N synthetase gene (pcbC) and consisted of four exons encoding an enzyme of 357 amino acids (approximately 40 kilodaltons [kDa]). The DNA coding sequences showed approximately 73% identity, while the amino acid sequences were approximately 76% identical. Noncoding DNA regions (including the region between pcbC and penDE) were not conserved. Acyltransferase activity from Escherichia coli producing the 40-kDa protein accepted either 6-aminopenicillanic acid or IPN as the substrate and made a penicillinase-sensitive antibiotic in the presence of phenylacetyl coenzyme A. Therefore, a single gene is responsible for converting IPN to p...Continue Reading

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Citations

Jan 25, 2011·Microbial Biotechnology·Juan F MartínCarlos García-Estrada
Jun 22, 2010·Nature Chemical Biology·Masanori FunabashiSteven G Van Lanen
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Marco Van Den BergIda Vander Keli
Nov 9, 2012·Natural Product Reports·Refaat B HamedChristopher J Schofield
Feb 7, 2003·Applied and Environmental Microbiology·Chi-Fai KimRichard Y C Kong
Jan 20, 2009·Eukaryotic Cell·Petra SpröteMichael J Hynes
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·A A Brakhage
May 28, 2009·BMC Microbiology·Carlos García-EstradaJuan Francisco Martín

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