Molecular characterization of the B-2 DNA puff gene of Rhynchosciara americana

Chromosoma
R V SantelliA J Stocker

Abstract

We have sequenced a 2.5-kb DNA fragment of the B-2 DNA puff from the sciarid Rhynchosciara americana and have defined its transcription unit. This puff is active during the formation of the communal cocoon, which is important for successful metamorphosis of this species and coincides with the final cycle of polytenization in its salivary glands. The B-2 polypeptide, together with the products of two other previously characterized DNA puffs, seems to be engaged in an interaction that results in a gradual modification and hardening of the cocoon structure. The B-2 messenger is temporally regulated in apparent coordination with the other puff products. The predicted polypeptide has characteristics similar to polypeptides from previously sequenced DNA puff genes, in particular those from the R. americana C-8 gene and the Bradysia hygida C-4 gene. The cloned sequence of the B-2 puff is differentially amplified in the three gland regions examined, achieving its highest amplification level of approximately fourfold (two extra cycles) in the anterior segment of the gland. The C-3 DNA puff sequence was also found to be differentially amplified in the different gland regions. Implications of the widespread presence of DNA amplification a...Continue Reading

Citations

Jun 6, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Paula Rezende-TeixeiraGláucia M Machado-Santelli
Aug 21, 2007·Genetica·Paula Rezende-TeixeiraGláucia M Machado-Santelli
Dec 24, 2010·Genome Research·Jared NordmanTerry L Orr-Weaver
May 8, 2010·Genetics and Molecular Research : GMR·P Rezende-TeixeiraG M Machado-Santelli
Sep 22, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alexandre de AndradeGlaucia Maria Machado-Santelli
May 17, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Juliana Aparecida Candido-SilvaJorge Cury de Almeida
Apr 8, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Paula Rezende-TeixeiraGlaucia Maria Machado-Santelli
Apr 28, 2006·Insect Molecular Biology·F SivieroR V Santelli
Nov 4, 2006·Genesis : the Journal of Genetics and Development·L R BassoM L Paçó-Larson
Jun 20, 2014·Arthropod Structure & Development·Amanda Dos Santos BrandãoGláucia Maria Machado-Santelli
May 15, 2016·Genesis : the Journal of Genetics and Development·Claudio Roberto SimonNadia Monesi
Oct 12, 2016·Genetics and Molecular Biology·Fábio SivieroGlaucia Maria Machado-Santelli
Apr 24, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J A Candido-Silva, N Monesi
Jan 16, 2008·Genesis : the Journal of Genetics and Development·Matheus S LecciNadia Monesi
Dec 14, 2007·Archives of Insect Biochemistry and Physiology·Luiz Paulo AndrioliJosé Mariano Amabis

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