Molecular characterization of the diversity of Clostridium chauvoei isolates collected from two bovine slaughterhouses: analysis of cross-contamination

Anaerobe
S Sathish, K Swaminathan

Abstract

Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rate disease affecting mainly cattle and sheep. Carcasses of animals affected by the disease are the chief source of soil infection and considered as an ever-present threat to livestock health. A study was undertaken to examine the cross-contamination of C. chauvoei in two different bovine slaughterhouses using restriction endonuclease analysis (REA) and protein analysis. Samples from various sites of two different bovine slaughterhouses were screened and 34 isolates were identified by conventional techniques and 16S rRNA gene (rrs) sequencing. C. chauvoei were isolated from carcass, soil, and sewage from slaughterhouses examined. The isolates were differentiated using REA and whole-cell and excretory protein pattern analysis combined with numerical analysis and cluster formation. The alpha and beta toxins produced by the strains were characterized. Our preliminary results suggest that REA combined with numerical analysis provides additional criteria and characteristic banding patterns for the study of the cross-contamination and characterization of C. chauvoei. The effects of temperature, oxygen tension, and enzymes on C. chauvoei hemolysin activity were...Continue Reading

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Citations

Mar 29, 2011·Applied and Environmental Microbiology·Alexander Rodriguez-PalaciosJeffrey T LeJeune
Feb 15, 2011·Veterinary Research·Edy M VileiJoachim Frey
Jan 3, 2013·International Journal of Food Microbiology·Leyre Lavilla LermaHikmate Abriouel
Oct 26, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Camila C AbreuFrancisco A Uzal
Feb 20, 2019·Veterinary Medicine and Science·Sonevilay NampanyaPeter A Windsor
Jun 11, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Camila C AbreuFrancisco A Uzal

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