Molecular characterization of the Drosophila melanogaster urate oxidase gene, an ecdysone-repressible gene expressed only in the malpighian tubules.

Molecular and Cellular Biology
L L WallrathT B Friedman

Abstract

The urate oxidase (UO) gene of Drosophila melanogaster is expressed during the third-instar larval and adult stages, exclusively within a subset of cells of the Malpighian tubules. The UO gene contains a 69-base-pair intron and encodes mature mRNAs of 1,224, 1,227, and 1,244 nucleotides, depending on the site of 3' endonucleolytic cleavage prior to polyadenylation. A direct repeat, 5'-AAGTGAGAGTGAT-3', is the proposed cis-regulatory element involved in 20-hydroxyecdysone repression of the UO gene. The deduced amino acid sequences of UO of D. melanogaster, rat, mouse, and pig and uricase II of soybean show 32 to 38% identity, with 22% of amino acid residues identical in all species. With use of P-element-mediated germ line transformation, 826 base pairs 5' and approximately 1,200 base pairs 3' of the D. melanogaster UO transcribed region contain all of the cis elements allowing for appropriate temporal regulation and Malpighian tubule-specific expression of the UO gene.

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A LepesantA Garen
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Apr 1, 1990·Molecular and Cellular Biology·A S LewinG M Small
Jul 11, 1985·Nucleic Acids Research·E B Keller, W A Noon
May 1, 1989·Journal of Molecular Evolution·E N Moriyama, T Gojobori
Nov 3, 1989·Cell·M Levine, J L Manley
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X W WuC T Caskey
Feb 10, 1989·Cell·M Beato
Feb 1, 1989·The Journal of Cell Biology·M Kozak
May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Aug 25, 1989·Nucleic Acids Research·I A Laird-OffringaA J van der Eb
Mar 22, 1985·Science·D J Lipman, W R Pearson
Apr 1, 1985·Cell·J E O'TousaM L Applebury
Jan 1, 1986·Gene·L G KralT B Friedman
Jun 20, 1986·Journal of Molecular Biology·L CherbasP Cherbas
Dec 1, 1986·Molecular and Cellular Biology·J HirshS B Scholnick
Aug 1, 1988·European Journal of Biochemistry·O Pongs
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P G ReddyN Usuda
Apr 15, 1988·European Journal of Biochemistry·M ItoY Takagi
Mar 5, 1988·Journal of Molecular Biology·H TakeshimaY Matsukado
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W I WoodR M Lawn
Jan 1, 1985·Annual Review of Cell Biology·P B Lazarow, Y Fujiki
Apr 1, 1985·Molecular and Cellular Biology·B MozerV Corces

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Citations

Nov 22, 2011·Journal of Insect Physiology·Dirk WeihrauchMichael J O'Donnell
Jul 1, 2004·Gene·Laura E NorwoodLori L Wallrath
Jan 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia Y ScaraffiaRoger L Miesfeld
Dec 1, 1991·Genomics·M ItoY Takagi
Jul 5, 2003·Physiological Reviews·Julian T Dow, Shireen A Davies
Aug 1, 2019·American Journal of Physiology. Renal Physiology·Saurav GhimireJulian A T Dow
Oct 9, 2020·G3 : Genes - Genomes - Genetics·Arthur LuhurAndrew C Zelhof
Jan 1, 1993·Insect Biochemistry and Molecular Biology·P Cherbas
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Patrick J GainesNancy Wisnewski

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