Molecular Characterization of the Epstein-Barr Virus BGLF2 Gene, its Expression, and Subcellular Localization

Iranian Journal of Biotechnology
Tao ChenMei-Li Li

Abstract

Epstein-Barr virus (EBV) is a universal herpes virus which can cause a life-long and largely asymptomatic infection in the human population. However, the exact pathogenesis of the EBV infection is not well known. A comprehensive bioinformatics prediction was carried out for investigating the molecular properties of the BGLF2 and to afford a foundation for future research of the role and instrument of BGLF2 in the course of EBV infection. A 1011-base-pair sequence of BGLF2 gene from the Epstein-Barr virus (EBV) Akata strain genome was amplified using polymerase chain reaction and was further characterized by cloning, sequencing, and subcellular localization in the COS-7 cells. The bioinformatics analysis demonstrated that EBV BGLF2 gene encodes a putative BGLF2 polypeptide which contains a conservative Herpes_UL16 domain. It was established that the polypeptide shows a close relationship with the Herpes UL16 tegument protein family and is extremely conserved among its homologues proteins encoded by UL16 genes. Multiple sequence alignments of the nucleic acid and amino acid sequence showed that the gene product of EBV BGLF2 contains a comparatively higher homology with the BGLF2-like proteins of the subfamily Gammaherpesvirinae t...Continue Reading

References

Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric JohannsenElliott Kieff
Nov 20, 2009·The Journal of General Virology·Bernhard EhlersDuncan J McGeoch
Jan 30, 2015·BioMed Research International·Pengjun ShiBin Yao
Jul 22, 2015·Archives of Virology·Meili LiMingsheng Cai
Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Oct 10, 2015·Archives of Biochemistry and Biophysics·Meili LiMingsheng Cai
Feb 9, 2016·Biological Chemistry·Mingsheng CaiMeili Li

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Citations

Jul 25, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tao ChenMingsheng Cai
Jan 11, 2022·PLoS Pathogens·Ashley M CampbellLori Frappier

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Datasets Mentioned

BETA
AAY41138

Methods Mentioned

BETA
PCR
transfection
electrophoresis
glycosylation
fluorescence microscopy

Software Mentioned

Clustal V
BLASTN
DNAstar
SignalP
nucleotide Basic Local Alignment Search Tool
Conserved
CPHmodels
NetNGlyc
FASTA
MegAlign

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