Molecular characterization of the genome-wide BOR transporter gene family and genetic analysis of BnaC04.BOR1;1c in Brassica napus

BMC Plant Biology
Haifei ChenF Xu

Abstract

Boron (B) deficiency is an agricultural problem that causes significant losses of crop yield in many areas of the world. However, systematic analysis of BOR family genes for B transport in rapeseed is still lacking. We aimed to identify and characterize BOR transporters in Brassica napus and the potential role of these transporters in B homeostasis in response to B deficiency. Here, we identified 20 BOR transporters from the Brassica napus genome, which were classified into six distinct groups that represent clear orthologous relationships to their family members in Arabidopsis. qRT-PCR revealed distinct expression profiles for BnBORs in different tissues and in response to external B levels. The B-efficient cultivar QY10 accumulated more B in shoots than the B-inefficient cultivar W10, and overexpression of BnaBOR1;1c could alleviate shoot B-deficiency symptoms in W10 by distributing more B from roots to shoots. Additionally, BnBOR1;1c expression was up-regulated by B deficiency, and the induction of BnBOR1;1c was more intense in QY10. Moreover, two conserved InDels were found in the promoter regions of BnBOR1;1c within different B-efficient genotypes. Overall, the molecular characterization of the BnBOR genes of two B-efficie...Continue Reading

References

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Citations

May 31, 2019·The Plant Journal : for Cell and Molecular Biology·Till Arvid DiehnGerd Patrick Bienert
Mar 27, 2020·International Journal of Molecular Sciences·Yuanyuan WanCunmin Qu
Feb 12, 2021·Plant & Cell Physiology·Amarachukwu Faith Onuh, Kyoko Miwa

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Methods Mentioned

BETA
transgenic
PCR
transmission electron microscopy

Software Mentioned

Multiple Expectation Maximization for Motif Elicitation ( MEME )
MEGA6
WebLogo
ExPASy
ClustalW2
MapInspect
BLASTP
BLAST
CNS
MEME

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