Molecular characterization of the glucose-sensing mechanism in the clonal insulin-secreting BRIN-BD11 cell line

Biochemical and Biophysical Research Communications
N H McClenaghanS Lenzen

Abstract

BRIN-BD11 cells represent a novel insulin-secreting cell line generated by electrofusion. Molecular characterization of these cells demonstrated the presence of mRNA and protein for the two key elements of the beta cell glucose-sensing system, GLUT2 and glucokinase. While levels of GLUT2 expression and 3-O-methyl-D-glucose equilibration were similar for both the BRIN-BD11 cell line and the parental control RINm5F cells, glucokinase expression was substantially higher in BRIN-BD11 cells. Expression of the two-component KATP channel complex, KIR6.2 and SUR1, was similar in both cells. However, while control RINm5F cells were completely unresponsive to glucose, BRIN-BD11 cells responded to physiological millimolar concentrations of this hexose sugar. These studies strongly suggest that the glucose-sensing ability of insulin-secreting cells is largely dictated by the level of glucokinase, as opposed to GLUT2, expression. Thus, BRIN-BD11 cells expressing the key attributes of the normal beta cell provide an interesting model for elucidation of regulatory principles of beta cell function.

References

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Citations

May 12, 2000·British Journal of Pharmacology·N H McClenaghanP R Flatt
Aug 26, 1998·Biochemical and Biophysical Research Communications·N H McClenaghanP R Flatt
Oct 6, 1999·Molecular and Cellular Endocrinology·A P SalgadoR Ramasamy

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