Abstract
BRIN-BD11 cells represent a novel insulin-secreting cell line generated by electrofusion. Molecular characterization of these cells demonstrated the presence of mRNA and protein for the two key elements of the beta cell glucose-sensing system, GLUT2 and glucokinase. While levels of GLUT2 expression and 3-O-methyl-D-glucose equilibration were similar for both the BRIN-BD11 cell line and the parental control RINm5F cells, glucokinase expression was substantially higher in BRIN-BD11 cells. Expression of the two-component KATP channel complex, KIR6.2 and SUR1, was similar in both cells. However, while control RINm5F cells were completely unresponsive to glucose, BRIN-BD11 cells responded to physiological millimolar concentrations of this hexose sugar. These studies strongly suggest that the glucose-sensing ability of insulin-secreting cells is largely dictated by the level of glucokinase, as opposed to GLUT2, expression. Thus, BRIN-BD11 cells expressing the key attributes of the normal beta cell provide an interesting model for elucidation of regulatory principles of beta cell function.
References
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W L ChickK C Kitchen
Apr 1, 1992·Cell Calcium·I Rustenbeck, S Lenzen
Jul 1, 1992·Diabetes·L I KoranyiM A Permutt
Nov 15, 1990·Analytical Biochemistry·R RagoG Wilding
Jun 1, 1990·Diabetes·F M Matschinsky
Oct 21, 1988·Cell·B ThorensH F Lodish
Feb 1, 1988·Biochemical Pharmacology·S Lenzen, U Panten
Jan 1, 1967·Diabetes·P E Lacy, M Kostianovsky
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A F GazdarV Lauris
Oct 1, 1983·Bioscience Reports·P RorsmanB Hellman
Nov 17, 1995·Science·N InagakiJ Bryan
Apr 21, 1995·Science·L Aguilar-BryanD A Nelson
Mar 17, 1995·The Journal of Biological Chemistry·N InagakiS Seino
May 1, 1994·Biochemical Society Transactions·N H McClenaghanP R Flatt
Feb 1, 1994·Biochemical Society Transactions·S Lenzen, M Tiedge
Jun 25, 1996·Biochemical and Biophysical Research Communications·N H McClenaghanP R Flatt
Aug 1, 1996·Diabetes·N H McClenaghanP R Flatt
Mar 1, 1996·The Journal of Endocrinology·N H McClenaghanP R Flatt
Dec 1, 1996·The Journal of Endocrinology·N H McClenaghanP R Flatt
Citations
May 12, 2000·British Journal of Pharmacology·N H McClenaghanP R Flatt
Feb 28, 2001·Diabetes, Obesity & Metabolism·N H McClenaghan, P R Flatt
Mar 10, 2001·Biochemical Pharmacology·N H McClenaghanP R Flatt
Jan 12, 2000·Pancreas·N H McClenaghan, P R Flatt
Nov 1, 2003·Endocrinology·Arnaud MerglenPierre Maechler
Feb 3, 2007·Experimental Physiology·Neville H McClenaghan
Oct 22, 2020·Open Biology·Petra DzianováLenka Žáková
Aug 26, 1998·Biochemical and Biophysical Research Communications·N H McClenaghanP R Flatt
Oct 6, 1999·Molecular and Cellular Endocrinology·A P SalgadoR Ramasamy
Jan 8, 1999·Biochimica Et Biophysica Acta·N H McClenaghan, P R Flatt
May 6, 2004·Cell Calcium·João C MiguelPeter R Flatt