Molecular characterization of the leopard gecko POMC gene and expressional change in the testis by acclimation to low temperature and with a short photoperiod

General and Comparative Endocrinology
Daisuke Endo, Min Kyun Park

Abstract

The gene for proopiomelanocortin (POMC), a common precursor of malanotropins, corticotropin, and beta-endorphin, was isolated and analyzed in the squamata species, the leopard gecko, Eublepharis macularius. Leopard gecko POMC (lgPOMC) cDNA is composed of 1299bp, excluding the poly(A) tail, and encodes 270 amino acids. The deduced amino acid sequence showed the same structural organization as that of other species and displayed identity with those of other vertebrates: 68% with mud turtles, 57/57% with African clawed frog A/B, 53% with chickens, and 45% with mice. In a phylogenic tree, the lgPOMC clustered with the sequences of the mud turtle POMC and python POMC. The lgPOMC gene comprises three exons and two introns and this structure is consistent with humans, rats, mice, African clawed frog and zebrafish. RT-PCR analysis revealed that the lgPOMC mRNA was expressed only in the whole brain, pituitary, and gonads. To analyze in more detail, a competitive assay system to quantify the expression levels of POMC mRNA was established. We measured the POMC mRNA expression levels in the leopard gecko testes following transfer from a condition of 29 degrees C, 16L/8D to 18 degrees C, 10L/14D over 6 weeks. This 6-week acclimation increas...Continue Reading

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Citations

May 17, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Daisuke Endo, Min Kyun Park
Jul 22, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Frank Seebacher
Mar 14, 2007·General and Comparative Endocrinology·Pezhman ShoureshiRobert M Dores
Mar 11, 2011·Annals of the New York Academy of Sciences·Robert M Dores, Andrea J Baron
Feb 7, 2006·General and Comparative Endocrinology·Ryno NaudéHiroshi Kawauchi
May 3, 2005·General and Comparative Endocrinology·Robert M Dores, Stephanie Lecaude
Sep 23, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Duk-Young Kang, Hyo-Chan Kim
Sep 15, 2005·American Journal of Medical Genetics. Part a·Orietta RadiGiovanna Camerino
Jan 10, 2019·Frontiers in Physiology·Colin G Scanes, Krystyna Pierzchala-Koziec
Feb 11, 2020·The Journal of Veterinary Medical Science·Hirohumi Suzuki, Toshiharu Yamamoto
Sep 30, 2019·General and Comparative Endocrinology·Ryan J Seddon, Diana K Hews
Jul 9, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ian A BouyoucosW Gary Anderson

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