PMID: 9190812Jun 1, 1997Paper

Molecular characterization of the mde operon involved in L-methionine catabolism of Pseudomonas putida

Journal of Bacteriology
H InoueH Tanaka

Abstract

A 15-kb region of Pseudomonas putida chromosomal DNA containing the mde operon and an upstream regulatory gene (mdeR) has been cloned and sequenced. The mde operon contains two structural genes involved in L-methionine degradative metabolism: the already-identified mdeA, which encodes L-methionine gamma-lyase (H. Inoue, K. Inagaki, M. Sugimoto, N. Esaki, K. Soda, and H. Tanaka. J. Biochem. (Tokyo) 117:1120-1125, 1995), and mdeB, which encodes a homologous protein to the homodimeric-type E1 component of pyruvate dehydrogenase complex. A rho-independent terminator was present just downstream of mdeB, and open reading frames corresponding to other components of alpha-keto acid dehydrogenase complex were not found. When MdeB was overproduced in Escherichia coli, the cell extract showed the E1 activity with high specificity for alpha-ketobutyrate rather than pyruvate. These results suggest that MdeB plays an important role in the metabolism of alpha-ketobutyrate produced by MdeA from L-methionine. Accordingly, mdeB encodes a novel E1 component, alpha-ketobutyrate dehydrogenase E1 component, of an unknown alpha-keto acid dehydrogenase complex in P. putida. In addition, we found that the mdeR gene was located on the opposite strand an...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 11, 1977·Biochemistry·H TanakaK Soda
May 1, 1991·Journal of Bacteriology·J A PalmerJ R Sokatch
Nov 1, 1991·The Biochemical Journal·B C Lockwood, G H Coombs
Jan 1, 1990·Annual Review of Biochemistry·S C Harrison, A K Aggarwal
Sep 11, 1989·FEBS Letters·C F HawkinsR N Perham
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I Saito, G R Stark
May 1, 1987·Molecular & General Genetics : MGG·T K Van Dyk, R A LaRossa
Jan 1, 1987·Methods in Enzymology·K Soda
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J BelfaizaI Saint-Girons
Jan 1, 1986·Annual Review of Biochemistry·T Platt
Jan 1, 1984·Molecular & General Genetics : MGG·A DanchinJ Daniel
May 1, 1984·Analytical Biochemistry·T NakayamaK Soda
Feb 1, 1995·Journal of Bacteriology·K T MadhusudhanJ R Sokatch
Nov 1, 1995·European Journal of Biochemistry·K HesterJ R Sokatch
May 1, 1996·European Journal of Biochemistry·S Hein, A Steinbüchel
Jun 14, 1996·Biochemical and Biophysical Research Communications·N HuangJ R Sokatch

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Citations

Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·B GrünenfelderU Jenal
Dec 21, 2010·Applied and Environmental Microbiology·Marie-Pierre ForquinIsabelle Martin-Verstraete
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hideaki KoikeMasashi Suzuki
Feb 2, 2008·Biological & Pharmaceutical Bulletin·Tsuyoshi KawashimaMasashi Suzuki
Oct 18, 2003·Journal of Bacteriology·Claudio AguilarVittorio Venturi
Oct 24, 2006·Journal of Bacteriology·Marie-Françoise HulloIsabelle Martin-Verstraete
Apr 26, 2020·Communications Biology·Zhi-Wei LiXiao-Chun Luo
Jan 17, 2007·Applied Microbiology and Biotechnology·Orianne CholetPascal Bonnarme
Dec 3, 2004·Applied and Environmental Microbiology·Felix AmaritaPascal Bonnarme

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