Molecular characterization of the PhoPQ-PmrD-PmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43.

Journal of Biomedical Science
Hsin-Yao ChengHwei-Ling Peng

Abstract

The cationic peptide antibiotic polymyxin has recently been reevaluated in the treatment of severe infections caused by gram negative bacteria. In this study, the genetic determinants for capsular polysaccharide level and lipopolysaccharide modification involved in polymyxin B resistance of the opportunistic pathogen Klebsiella pneumoniae were characterized. The expressional control of the genes responsible for the resistance was assessed by a LacZ reporter system. The PmrD connector-mediated regulation for the expression of pmr genes involved in polymyxin B resistance was also demonstrated by DNA EMSA, two-hybrid analysis and in vitro phosphor-transfer assay. Deletion of the rcsB, which encoded an activator for the production of capsular polysaccharide, had a minor effect on K. pneumoniae resistance to polymyxin B. On the other hand, deletion of ugd or pmrF gene resulted in a drastic reduction of the resistance. The polymyxin B resistance was shown to be regulated by the two-component response regulators PhoP and PmrA at low magnesium and high iron, respectively. Similar to the control identified in Salmonella, expression of pmrD in K. pneumoniae was dependent on PhoP, the activated PmrD would then bind to PmrA to prolong the ...Continue Reading

References

Aug 15, 1994·Annals of the New York Academy of Sciences·P DomenicoB A Cunha
Feb 22, 1996·Gene·K Skorupski, R K Taylor
Feb 8, 1997·Lancet·R E Hancock
Mar 21, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E A Groisman
Apr 1, 1997·Advances in Dental Research·Y KeisariI Ofek
Sep 10, 1999·The Journal of Biological Chemistry·M M Wösten, E A Groisman
Oct 29, 2000·Cell·M M WöstenE A Groisman
Feb 27, 2001·Journal of Bacteriology·E A Groisman
Jan 10, 2003·Molecular Microbiology·Chakib Mouslim, Eduardo A Groisman
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Akinori KatoEduardo A Groisman
Jun 19, 2003·Journal of Bacteriology·Shu MinagawaRyutaro Utsumi
Nov 24, 2004·Infection and Immunity·Miguel A CamposJosé A Bengoechea
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mollie D Winfield, Eduardo A Groisman
Feb 16, 2005·The Journal of Biological Chemistry·Mollie D WinfieldEduardo A Groisman
Sep 13, 2005·Annual Review of Microbiology·Nadim Majdalani, Susan Gottesman
Feb 3, 2006·The Journal of Infectious Diseases·Yi-Ping ChuangJin-Town Wang
Jan 19, 2007·Molecular Microbiology·J Christian Perez, Eduardo A Groisman
Feb 20, 2007·International Journal of Antimicrobial Agents·Matthew E Falagas, Ioannis A Bliziotis
Sep 1, 2007·Current Opinion in Critical Care·Matthew E Falagas, Petros Kopterides
Sep 20, 2007·The Journal of Antimicrobial Chemotherapy·Alexandre Prehn ZavasckiRoger L Nation
Oct 12, 2007·The Journal of Biological Chemistry·Aixin YanChristian R H Raetz
Oct 25, 2008·PLoS Genetics·Alexander Y MitrophanovEduardo A Groisman
Apr 17, 2009·Nature Reviews. Microbiology·Ronald S FlannaganSergio Grinstein

❮ Previous
Next ❯

Citations

May 24, 2012·The Journal of Antimicrobial Chemotherapy·Keith Poole
Aug 17, 2011·Antimicrobial Agents and Chemotherapy·Bruk MensaWilliam F DeGrado
Jun 27, 2013·Annual Review of Microbiology·H Deborah Chen, Eduardo A Groisman
Mar 1, 2012·PloS One·Baldiri SalvadoRui Alves
Jul 18, 2012·Critical Reviews in Microbiology·José Luis Anaya-LópezAlejandra Ochoa-Zarzosa
Oct 22, 2015·Expert Review of Anti-infective Therapy·Yiying CaiAndrea L Kwa
Jan 15, 2014·Diagnostic Microbiology and Infectious Disease·Ji-Young Lee, Kwan Soo Ko
Mar 19, 2016·Scientific Reports·Mohit KumarEnketeswara Subudhi
Apr 24, 2015·The Journal of Antimicrobial Chemotherapy·Matthew E WandJ Mark Sutton
May 20, 2015·International Journal of Antimicrobial Agents·Aurélie JayolPatrice Nordmann
Sep 26, 2014·Current Opinion in Pharmacology·Gian Maria RossoliniSimona Pollini
Dec 17, 2014·Frontiers in Microbiology·Abiola O OlaitanJean-Marc Rolain
May 4, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M S RamirezM E Tolmasky
Sep 6, 2014·The Journal of Antimicrobial Chemotherapy·Laurent PoirelPatrice Nordmann
Mar 6, 2018·The Journal of Antimicrobial Chemotherapy·Yi-Hsiang ChengJin-Town Wang
Dec 13, 2017·Antimicrobial Agents and Chemotherapy·Nadia JaidaneThierry Naas
Mar 8, 2018·The Journal of Antimicrobial Chemotherapy·Sue C NangJian Li
Feb 19, 2015·Antimicrobial Agents and Chemotherapy·Yi-Hsiang ChengJin-Town Wang
Feb 19, 2015·Antimicrobial Agents and Chemotherapy·Antonio CannatelliGian Maria Rossolini
Mar 4, 2015·Antimicrobial Agents and Chemotherapy·Aurélie JayolLaurent Poirel
Apr 14, 2016·Antimicrobial Agents and Chemotherapy·Yi-Hsiang ChengJin-Town Wang
Nov 18, 2018·The Journal of Antimicrobial Chemotherapy·Miranda E PittMatthew A Cooper
May 9, 2019·Future Microbiology·Aimee Rp Tierney, Philip N Rather
May 28, 2020·Antimicrobial Agents and Chemotherapy·Ching Hei Phoebe CheungMatthew B Avison
Aug 10, 2016·Cold Spring Harbor Perspectives in Medicine·Michael J TrimbleRobert E W Hancock
Apr 13, 2019·International Journal of Molecular Sciences·Anjali Y BhagirathKangmin Duan

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Methods Mentioned

BETA
PCR
electrophoretic mobility shift assay
electrophoresis
two-hybrid
phosphotransfer assay
2-hybrid

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