Molecular characterization of the processing of arenavirus envelope glycoprotein precursors by subtilisin kexin isozyme-1/site-1 protease.

Journal of Virology
Dominique J BurriStefan Kunz

Abstract

A crucial step in the life cycle of arenaviruses is the biosynthesis of the mature fusion-active viral envelope glycoprotein (GP) that is essential for virus-host cell attachment and entry. The maturation of the arenavirus GP precursor (GPC) critically depends on proteolytic processing by the cellular proprotein convertase (PC) subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P). Here we undertook a molecular characterization of the SKI-1/S1P processing of the GPCs of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) and the pathogenic Lassa virus (LASV). Previous studies showed that the GPC of LASV undergoes processing in the endoplasmic reticulum (ER)/cis-Golgi compartment, whereas the LCMV GPC is cleaved in a late Golgi compartment. Herein we confirm these findings and provide evidence that the SKI-1/S1P recognition site RRLL, present in the SKI-1/S1P prodomain and LASV GPC, but not in the LCMV GPC, is crucial for the processing of the LASV GPC in the ER/cis-Golgi compartment. Our structure-function analysis revealed that the cleavage of arenavirus GPCs, but not cellular substrates, critically depends on the autoprocessing of SKI-1/S1P, suggesting differences in the processing of cellular and viral sub...Continue Reading

References

Aug 1, 1983·The Journal of General Virology·F J Dutko, M B Oldstone
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·O LenzW Garten
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·J YangJ D Horton
Jan 5, 2002·The Journal of Biological Chemistry·Aram ElagozNabil G Seidah
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 26, 2002·American Journal of Obstetrics and Gynecology·Leslie L BartonCynthia L Beauchamp
Dec 31, 2002·Virology·Angela M Archer, Rebeca Rico-Hesse
Jul 30, 2003·Journal of Virology·Martin J VincentStuart T Nichol
Jun 15, 2005·Annual Review of Biochemistry·Martin Schröder, Randal J Kaufman
May 26, 2006·The New England Journal of Medicine·Staci A FischerUNKNOWN LCMV in Transplant Recipients Investigation Team
Jul 12, 2007·The Journal of Biological Chemistry·Philomena PullikotilNabil G Seidah
Feb 8, 2008·The New England Journal of Medicine·Gustavo PalaciosW Ian Lipkin
Dec 9, 2009·Advances in Pediatrics·Daniel J Bonthius
Nov 26, 2010·Journal of Virology·Giulia PasqualStefan Kunz

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Citations

Dec 4, 2012·Viruses·Shuzo Urata, Jiro Yasuda
Dec 4, 2012·Viruses·Dominique J BurriAntonella Pasquato
Oct 1, 2013·Journal of Molecular Biology·Jill M PerreiraAbraham L Brass
Feb 18, 2016·Expert Opinion on Drug Discovery·Antonella Pasquato, Stefan Kunz
Apr 25, 2013·Antiviral Research·Antonella PasquatoStefan Kunz
Aug 27, 2016·Virologica Sinica·Wei WangGengfu Xiao
Dec 22, 2016·MBio·Punya Shrivastava-RanjanChristina F Spiropoulou
Dec 18, 2012·Expert Review of Anti-infective Therapy·Antonella PasquatoStefan Kunz
Dec 10, 2015·The Journal of Biological Chemistry·Joel Ramos da PalmaStefan Kunz
Nov 8, 2014·The Journal of Biological Chemistry·Joel Ramos da PalmaAntonella Pasquato

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