Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster

Molecular Microbiology
L L BurrowsJ S Lam

Abstract

Pseudomonas aeruginosa co-expresses A-band lipopolysaccharide (LPS), a homopolymer of rhamnose, and B-band LPS, a heteropolymer with a repeating unit of 2-5 sugars which is the serotype-specific antigen. The gene clusters for A- and B-band biosynthesis in P. aeruginosa O5 (strain PAO1) have been cloned previously. Here we report the DNA sequence and molecular analysis of the B-band O-antigen biosynthetic cluster. Sixteen open reading frames (ORFs) thought to be involved in synthesis of the O5 O antigen were identified, including wzz (rol), wzy (rfc), and wbpA-wbpN. A further 3 ORFs not thought to be involved with LPS synthesis were identified (hisH, hisF, and uvrB). Most of the wbp genes are found only in serotypes O2, O5, O16, O18, and O20, which form a chemically and structurally related O-antigen serogroup. In contrast, wbpM and wbpN are common to all 20 serotypes of P. aeruginosa. Although wbpM is not serogroup-specific, knockout mutations confirmed it is necessary for O5 O-antigen biosynthesis. A novel insertion sequences, IS 1209, is present at the junction between the serogroup-specific and non-specific regions. We have predicted the functions of the proteins encoded in the wbp cluster based on their homologies to those ...Continue Reading

Citations

Jan 22, 2003·Environmental Microbiology·Craig DanielsJoseph S Lam
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael J CoyneLaurie E Comstock
Jul 8, 2010·The Journal of Biological Chemistry·Tae-Hyun KimDennis L Kasper
Aug 29, 2007·Bioinformatics·Issaac RajanSharmila S Mande
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Julie M Struble, Ryan T Gill
Nov 28, 2012·Applied and Environmental Microbiology·Jessica A SmithPier-Luc Tremblay
Feb 2, 2010·Journal of Bacteriology·Thilo KöhlerChristian van Delden
Aug 2, 2011·Journal of Bacteriology·Michael J CoyneLaurie E Comstock
Jun 12, 2002·Journal of Bacteriology·Christopher K RaymondMaynard V Olson
Oct 31, 2002·Molecular Microbiology·Antonio DiGiandomenicoPeter Castric
Nov 2, 2014·Canadian Journal of Microbiology·Salim T Islam, Joseph S Lam
Nov 25, 2004·Environmental Microbiology·V A P Martins Dos SantosK N Timmis

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