Molecular Characterization of the Recently Emerged Poultry Pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing

PloS One
Susann ThiemeH M Hafez

Abstract

Ornithobacterium rhinotracheale (ORT) is an economically important bacterial pathogen of turkeys and chickens worldwide. Since its first detection, a variety of typing methods have been used to gain basic knowledge about the bacterial population structure, an issue that still needs to be addressed. Serological characterization revealed at least 18 different serotypes (A-R) with ORT of serotype A to be predominate among poultry. This study aimed to establish a multilocus sequence typing (MLST) scheme for ORT that could easily be used by other laboratories and allows for worldwide comparison of sequence data. For this purpose, 87 ORT strains from different poultry hosts, geographical origins, years of isolation and serotypes were included in the analysis to identify correlations. Fourteen different sequence types (ST) were found. The most common ST1 was identified in 40 ORT strains from turkeys and chickens on 4 continents and in 3 different European countries. Together with ST9, both STs represented over three quarters (77%) of ORT strains used in the MLST analysis and included strains of frequently cross-reacting ORT serotypes A, E and I. Nine STs were only represented by one ORT strain and might indicate possible avian host, d...Continue Reading

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Citations

Apr 20, 2017·Avian Pathology : Journal of the W.V.P.A·Réka SzabóTibor Magyar
Apr 13, 2018·The Journal of Veterinary Medical Science·Roberto Montes DE Oca-JimenezEdgardo Soriano-Vargas
Apr 5, 2020·Applied and Environmental Microbiology·Emily A SmithTimothy J Johnson
Feb 12, 2021·Avian Diseases·Inês Berenguer VeigaDörte Lüschow

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Methods Mentioned

BETA
electrophoresis
ribotyping
PCR

Software Mentioned

Primer
MEGA
MLST
Chromas Lite

Related Concepts