PMID: 3747520Aug 1, 1986Paper

Molecular characterization of the sex steroid binding protein (SBP) of plasma. Re-examination of rabbit SBP and comparison with the human, macaque and baboon proteins

Journal of Steroid Biochemistry
P H PétraJ B Ross

Abstract

Physico-chemical characterization of the sex steroid-binding protein, SBP, of rabbit plasma reveals that it is a dimer of mol. wt 85,800 composed of similar subunits of mol. wt 43,000. These data confirm our original proposal for a dimeric structure. The protein contains 9% carbohydrate, comprised of mannose, galactose, N-acetylglucosamine and sialic acid. It is devoid of N-acetylgalactosamine and fucose. The protein binds one molecule of 5 alpha-dihydrotestosterone per dimer with a Kd of 0.89 nM (12 degrees C). Comparison with the human, monkey and baboon SBPs indicates that all these proteins have the same dimeric molecular organization and exhibit microheterogeneity in SDS-PAGE and isoelectricfocusing. Rabbit SBP, however, contains less carbohydrate and has a higher polypeptide molecular weight than all the other SBPs. Spectrophotometric data also indicate that some tryptophan residues are in a different chemical environment than those in other SBPs. The observed microheterogeneity in all four SBP species is due for the most part to variable glycosylation of the subunit and variability at the amino-terminal region of the subunit. Combination of these and other phenomena will generate a significant number of isomeric forms of...Continue Reading

References

Apr 14, 1976·Biochimica Et Biophysica Acta·A Grinvald, I Z Steinberg
Mar 11, 1975·Biochemistry·K E Mickelson, P H Pétra
Nov 14, 1978·Biochemical and Biophysical Research Communications·S BordinP H Petra
May 1, 1979·Journal of Steroid Biochemistry·M J Iqbal, M W Johnson
Jan 1, 1986·Journal of Steroid Biochemistry·P H PetraK Titani
Nov 1, 1972·Analytical Biochemistry·D J DeRosierD J Cox
Nov 7, 1969·Annals of the New York Academy of Sciences·D E Roark, D A Yphantis
Jun 1, 1969·Biochemistry·C E HarrisW J Rutter
Oct 1, 1983·Journal of Steroid Biochemistry·C Y ChengC W Bardin
Jan 1, 1981·Annual Review of Physiology·C W BardinR Becker

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Citations

Dec 1, 1994·Journal of Fluorescence·S BenciP Vaghi
Jul 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·M P AuzelleH Degrelle
Jan 1, 1986·Journal of Steroid Biochemistry·P H PetraK Titani
Jan 1, 1987·Journal of Steroid Biochemistry·C Mercier-BodardE E Baulieu
Apr 1, 1990·Journal of Steroid Biochemistry·O A Strel'chyonok, G V Avvakumov
Jan 1, 1995·Neuroscience and Biobehavioral Reviews·D A Damassa, J M Cates
Mar 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D A DamassaA W Gustafson
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·S GershagenC M Edenbrandt
Oct 26, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D KouretasO Antonoglou
Jan 6, 2000·The Journal of Steroid Biochemistry and Molecular Biology·P CousinM Pugeat
Mar 9, 2000·Nuclear Medicine and Biology·T J TewsonP Petra
Jan 1, 1991·Annals of the New York Academy of Sciences·A GerardH Gerard
Sep 23, 2014·Molecular and Cellular Endocrinology·Mikhail N ZakharovRavi Jasuja
Jan 30, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Bela BendlováJana Vrbíková

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