PMID: 9442102Feb 28, 1998Paper

Molecular characterization of the SUMO-1 modification of RanGAP1 and its role in nuclear envelope association

The Journal of Cell Biology
R MahajanFrauke Melchior

Abstract

The mammalian guanosine triphosphate (GTP)ase-activating protein RanGAP1 is the first example of a protein covalently linked to the ubiquitin-related protein SUMO-1. Here we used peptide mapping, mass spectroscopy analysis, and mutagenesis to identify the nature of the link between RanGAP1 and SUMO-1. SUMO-1 is linked to RanGAP1 via glycine 97, indicating that the last 4 amino acids of this 101- amino acid protein are proteolytically removed before its attachment to RanGAP1. Recombinant SUMO-1 lacking the last four amino acids is efficiently used for modification of RanGAP1 in vitro and of multiple unknown proteins in vivo. In contrast to most ubiquitinated proteins, only a single lysine residue (K526) in RanGAP1 can serve as the acceptor site for modification by SUMO-1. Modification of RanGAP1 with SUMO-1 leads to association of RanGAP1 with the nuclear envelope (NE), where it was previously shown to be required for nuclear protein import. Sufficient information for modification and targeting resides in a 25-kD domain of RanGAP1. RanGAP1-SUMO-1 remains stably associated with the NE during many cycles of in vitro import. This indicates that removal of RanGAP1 from the NE is not a required element of nuclear protein import and s...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jan 1, 1992·Methods in Enzymology·S A AdamL Gerace
Jul 1, 1989·Molecular and Cellular Biology·H M TragliaA K Hopper
Dec 1, 1994·Molecular and Cellular Biology·K R Loeb, A L Haas
Jul 13, 1995·Nature·N YokoyamaU Aebi
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
Jul 1, 1995·European Journal of Biochemistry·H P Jennissen
Sep 1, 1995·The Journal of Cell Biology·A H CorbettP A Silver
Jun 1, 1995·Current Opinion in Cell Biology·F Melchior, L Gerace
Mar 1, 1995·Molecular and Cellular Biology·J SpenceD Finley
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·F R BischoffH Ponstingl
Oct 7, 1994·Cell·A Ciechanover
Jan 1, 1994·International Review of Cytology·C M Feldherr, D Akin
Aug 31, 1993·Biochemical and Biophysical Research Communications·S KumarM Noda
Mar 15, 1996·Science·D Görlich, I W Mattaj
May 6, 1996·Biochemical and Biophysical Research Communications·H MannenS S Li
Oct 3, 1996·Biochemical and Biophysical Research Communications·H CaoN S Chang
Mar 1, 1996·Trends in Biochemical Sciences·W Hilt, D H Wolf
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser

❮ Previous
Next ❯

Citations

May 16, 2002·Journal of Cellular Physiology·Keun Il KimChin Ha Chung
Oct 13, 2006·Chromosoma·Felicity Z Watts
Oct 30, 2001·Virus Research·V G Wilson, D Rangasamy
May 12, 2000·Gene·E T YehT Kamitani
Apr 12, 2003·Brain & Development·Hatice Asuman Ozkara, Konrad Sandhoff
Mar 1, 2000·Trends in Biochemical Sciences·W M Gray, I Estelle
Sep 12, 1998·Molecular Cell·J M DesterroR T Hay
May 14, 1999·Trends in Plant Science· del Pozo JC, M Estelle
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S ApionishevR S Rasooly
Oct 13, 2000·European Journal of Biochemistry·T NishidaH Yasuda
Apr 15, 2010·The Journal of Biological Chemistry·Henri A BlomsterLea Sistonen
May 27, 2010·The Journal of Biological Chemistry·Jianghai Wang, Yuan Chen
Aug 6, 2010·The Journal of Biological Chemistry·Keally J MirandaRaghunatha R Yammani
Dec 4, 2004·Molecular & Cellular Proteomics : MCP·Germán Rosas-AcostaVan G Wilson
Nov 23, 2005·The Journal of Experimental Medicine·Honglin ZhouVishva M Dixit
May 8, 1999·Molecular Biology of the Cell·I TanidaE Kominami
Oct 11, 2003·Molecular Biology of the Cell·April S GoehringGeorge F Sprague
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R T HayM S Rodriguez
Jun 5, 2003·The Plant Cell·Luisa Maria LoisNam-Hai Chua
Oct 14, 2003·Eukaryotic Cell·April S GoehringGeorge F Sprague
Aug 23, 2002·Molecular and Cellular Biology·Hong ZhangMichael J Matunis
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Oct 14, 2000·Annual Review of Cell and Developmental Biology·F Melchior
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A J van BrabantN A Ellis
Feb 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fangting WuYin-Yuan Mo
Feb 16, 2005·BMC Developmental Biology·Phoebe L StewartKathy A Suprenant
Feb 26, 2009·International Journal of Biological Sciences·Yanisa Laoong-u-thaiJinzeng Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.