Abstract
T lymphocytes play a central role in the pathogenesis of a large number of human conditions including autoimmunity and graft rejection. Although T cells are key players in mounting immune responses, the assessment of T cell repertoires has yet to find an important role in clinical decision making. In this review, we discuss the "immunoscope" technique and its potential diagnostic role in a variety of clinical scenarios. This is an RT-PCR based approach that subdivides a bulk T cell population (i. e. from blood, lymph, spleen, or tissue) into approximately 2800 groups based upon rearranged variable beta (Vbeta)/joining beta (Jbeta) gene segments and the resulting length of the T cell receptor's (TCR's) third complementarity determining region (CDR-3). This extensive subdivision, or focusing, allows clonal expansions to be directly observed. Such a fine-tuned analysis has revealed previously unappreciated aspects of the T cell repertoire. For instance, an antigen-specific immune response can be divided into both public and non-public components. The non-public repertoire contains the majority of the expanding T cells which are unique to the individual (private), or shared by only some (semi-private), while "public" T cells can be...Continue Reading
Citations
Nov 22, 2005·Clinical & Developmental Immunology·Mathilda MandelAnat Achiron
Dec 20, 2012·Antioxidants & Redox Signaling·Jared ManningGerald J Spangrude
Apr 12, 2011·Clinical and Molecular Allergy : CMA·Roshini S Abraham
Jul 21, 2010·PloS One·Maria Cristina De RosaFrancesco Ria
Feb 15, 2013·PloS One·Chiara NicolòFrancesco Ria
May 15, 2012·Molecular Immunology·Idania MarreroJoanna D Davies
Jan 31, 2012·Cellular Immunology·Emanual Maverakis
Jul 2, 2014·Journal of Immunological Methods·Peter J WettsteinNeil E Kay
Aug 26, 2006·Molecular Immunology·Patrick MiqueuSophie Brouard
Jun 25, 2015·Memórias do Instituto Oswaldo Cruz·Raquel FerrazAlvaro Luiz Bertho
Feb 7, 2018·Frontiers in Immunology·Aurélie DurgeauFathia Mami-Chouaib
Nov 18, 2006·Journal of Leukocyte Biology·Paolo SommaMaria Teresa Fiorillo
Dec 6, 2006·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Alison J QuayleAnn Duerr
Sep 1, 2005·Expert Review of Clinical Immunology·Rémi J CreusotIngo H Tarner
Jul 13, 2018·JCI Insight·Alexander A MerleevEmanual Maverakis
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shane A CurranRobert Winchester
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tania PannelliniPiero Musiani
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne M Ercolini, Stephen D Miller
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simona RollaFrancesco Ria
Nov 24, 2007·Seminars in Immunology·Lori L HudsonGregory D Sempowski