Molecular characterization of thyroid peroxidase gene in porcine (Sus scrofa)

Journal of Genetics and Genomics = Yi Chuan Xue Bao
Ying WangNingying Xu

Abstract

Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Several mutations in the TPO gene may affect the normal growth and development of mammals. In this study, the TPO gene was mapped, its expression level was determined in thyroid grand at the age of 1, 20, 45, 60, 90, 120 and 150 days for Jinhua pig, the alternative splicing form was searched and the single nucleotide polymorphism (SNP, A/G(642)) of TPO gene was analyzed. The results showed that the porcine TPO was mapped to 3q22-27, the expression level of TPO was relatively stable among the various ages and two novel transcript variants in porcine TPO gene were found: the splicing variant TPO-2 lacked exon 8, while TPO-3 lacked exon 8 and exon 14, 15, 16. Moreover, we found that the SNP of A/G(642) in the fourteenth exon of TPO gene was significantly associated with ham weight (P < 0.05). Our results provided important basis on the regulation and metabolism of the thyroid gland in animals.

References

Jul 31, 1990·Biochemical and Biophysical Research Communications·E ZanelliY Malthièry
Nov 1, 1987·Molecular Endocrinology·R P MagnussonB Rapoport
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S KimuraS Ohtaki
Aug 2, 1994·Biochimica Et Biophysica Acta·G Damante, R Di Lauro
Jun 11, 1998·Endocrine Journal·F Kambe, H Seo
Jul 14, 1999·Biochimie·A Taurog
Mar 16, 2000·Thyroid : Official Journal of the American Thyroid Association·C De MiccoM Grino
Jan 26, 2002·Animal Biotechnology·A A PaszekL B Schook
Jul 13, 2002·Endocrine Pathology·Marcus A. LimaFernando C. L. Schmitt
May 1, 2007·European Journal of Endocrinology·Magdalena AvbeljTadej Battelino

❮ Previous
Next ❯

Citations

Jan 1, 2013·Molecular Biology Reports·Xinyan HouXiangtao Kang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.