Molecular characterization of triosephosphate isomerase from Echinococcus granulosus

Parasitology Research
Maodi WuGuangyou Yang

Abstract

Cystic echinococcosis (CE) is a zoonosis that can be caused by the larvae of Echinococcus granulosus; this disease occurs worldwide and is highly endemic in China. E. granulosus can produce energy by glycolysis as well as both aerobic and anaerobic respirations. Triosephosphate isomerase is a glycolytic enzyme present in a wide range of organisms and plays an important role in glycolysis. However, there has been little research on triosephosphate isomerase from E. granulosus (Eg-TIM). Here, we present a bioinformatic characterization and the experimentally determined tissue distribution characteristics of Eg-TIM. We also explored its potential value for diagnosing CE in sheep using indirect enzyme-linked immunosorbent assay (ELISA). Native Eg-TIM was located in the neck and hooks of protoscoleces (PSCs), as well as the tegument and parenchyma tissue of adult worms. The entire germinal layer was also Eg-TIM positive. Western blots showed that recombinant Eg-TIM (rEg-TIM) reacts with positive serum from sheep and had good immunogenicity. Indirect ELISA exhibited low specificity (53.6%) and low sensitivity (87.5%) and cross-reacted with both Taenia multiceps and Taenia hydatigena. Our results suggest that TIM may take part in the ...Continue Reading

References

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Citations

Dec 4, 2021·Frontiers in Cellular and Infection Microbiology·Kuerbannisha AmahongGuodong Lü

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Datasets Mentioned

BETA
CDS23836.1
CDS36936.1
AAG21132.1
CDS29962.1
GAA50994
AAA29919.1
AAC47393

Methods Mentioned

BETA
ELISA
Fluorescence

Software Mentioned

ExPaSy
Eg
TMHMM
SignalP
SPSS Statistics
TIM
MEGA

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