Molecular Characterization of Twenty-Five Marine Cyanobacteria Isolated from Coastal Regions of Ireland

Biology
Katie ShielsSushanta Kumar Saha

Abstract

Twenty-five marine cyanobacteria isolated from Irish coasts were characterized based on their morphological characters and 16S rRNA gene sequence analysis. In addition, superoxide dismutase (SOD) and malate dehydrogenase (MDH) isoenzyme banding patterns were used to differentiate two morphologically ambiguous isolates. In this study, six new cyanobacteria-specific primers were designed, and a 16S rRNA gene of twenty-five morphologically diverse cyanobacteria was successfully PCR amplified (1198-1396 bps). Assembled 16S rRNA sequences were used both for a basic local alignment search tool (BLAST) analysis for genus-level identification and to generate a phylogenetic tree, which yielded two major clusters: One with morphologically homogenous cyanobacteria and the other with morphologically very diverse cyanobacteria. Kamptonemaokenii and Tychonemadecoloratum were isolated from a single field sample of Ballybunion and were originally identified as the same 'Oscillatoria sp.' based on preliminary morphological observations. However, an alignment of 16S rRNA gene sequences and SOD and MDH isoenzyme banding pattern analyses helped in differentiating the morphologically-indistinguishable 'Oscillatoria sp.'. Finally, after a re-evaluat...Continue Reading

References

Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Aug 1, 1997·Applied and Environmental Microbiology·U NübelG Muyzer
Jan 29, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T Kondo, M Ishiura
Apr 20, 2002·Medical and Veterinary Entomology·F A MonteiroA M Solé-Cava
Oct 31, 2002·Applied and Environmental Microbiology·Maria J LaamanenKaarina Sivonen
Nov 29, 2007·BMC Genomics·Balakrishnan PriyaGopalakrishnan Subramanian
Jun 1, 2002·FEMS Microbiology Ecology·Weiwen ZhengUlla Rasmussen
Aug 1, 2003·FEMS Microbiology Ecology·Sushanta Kumar SahaGopalakrishnan Subramanian
May 9, 2014·Water Research·Elvina LeeAndrea Paparini
Feb 24, 2016·Molecular Phylogenetics and Evolution·Panos BravakosAthena Economou-Amilli
Mar 11, 2017·Molecular Phylogenetics and Evolution·Ângela BritoPaula Tamagnini
Nov 5, 2017·Systematic and Applied Microbiology·Hang T L PhamSabine Mundt

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Datasets Mentioned

BETA
SABC022701
SABC022801
SABC022901
SABC011901

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

BioEdit
Gblocks
MUSCLE
Clustal Omega
Total Lab TL100
PhyML
BLAST
TreeDyn

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