Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis.

Journal of Clinical Microbiology
D N CameronT J Barrett

Abstract

Pulsed-field gel electrophoresis (PFGE) was performed on 180 isolates of Vibrio cholerae serogroup O1 representing 6 different multilocus enzyme electrophoresis (MEE) types and 27 rRNA restriction fragment length polymorphism types (ribotypes). Isolates were digested with the restriction enzyme NotI and were separated into 63 patterns on the basis of differences in band arrangements. In general, strains which were different by MEE or ribotyping also had different PGFE patterns. PFGE identified individual strains within a single MEE type or ribotype; isolates with one PFGE pattern were less frequently distinguished by ribotyping. All V. cholerae O1 isolates tested from the Latin American epidemic were indistinguishable by their MEE, ribotype, or PFGE patterns. PFGE could further distinguish strains of this same ribotype isolated in Africa, Europe, the South Pacific, or Southeast Asia. Although both MEE and PFGE could identify the strain from the Latin American epidemic, PFGE was more rapid and less labor intensive. PFGE also distinguished nontoxigenic isolates endemic to the U.S. Gulf Coast from unrelated nontoxigenic isolates. In the present study PFGE was more discriminating than other previously described subtyping assays for...Continue Reading

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Citations

Apr 21, 2012·Journal of Clinical Microbiology·Munirul AlamAlejandro Cravioto
Feb 12, 2013·Current Microbiology·Abolfazl Dashtbani-RoozbehaniMohammad Reza Pourshafie
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Munirul AlamAlejandro Cravioto
Aug 26, 2016·Clinical Microbiology Reviews·J RonholmFranco Pagotto
Nov 11, 2017·Science·Daryl DommanNicholas R Thomson
Dec 13, 2006·Asia-Pacific Journal of Public Health·L Iyer, J Vadivelu
Sep 11, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Melissa Tobin-D'AngeloEric Mintz
Oct 27, 2015·Frontiers in Microbiology·Md Habibur RahamanMunirul Alam
Oct 23, 2013·The Journal of Infectious Diseases·Rituparna DeG Balakrish Nair
Apr 8, 2011·Emerging Infectious Diseases·Afsar AliJ Glenn Morris
Jun 29, 2011·Letters in Applied Microbiology·A Dashtbani-RoozbehaniM R Pourshafie
Nov 18, 2000·Infection and Immunity·O C StineJ A Johnson
Feb 22, 2001·Journal of Travel Medicine·H J KoornhofL McGee

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