Molecular characterization of vitellogenin and its receptor genes from citrus red mite, Panonychus citri (McGregor)

International Journal of Molecular Sciences
Rui ZhongJin-Jun Wang

Abstract

The production and uptake of yolk protein play an important role in the reproduction of all oviparous organisms. Vitellogenin (Vg) is the precursor of vitellin (Vn), which is the major egg storage protein, and vitellogenin receptor (VgR) is a necessary protein for the uptake of Vg into developing oocytes. In this paper, we characterize the full-length Vg and VgR, PcVg1 and PcVgR, respectively, of the citrus red mite Panonychus citri (McGregor). The PcVg1 cDNA is 5748 nucleotides (nt) with a 5553-nt open reading frame (ORF) coding for 1851 amino acids (aa), and the PcVgR is 6090 nt, containing an intact ORF of 5673 nt coding an expected protein of 1891 aa. The PcVg1 aa sequence shows a typical GLCG domain and several K/RXXR cleavage sites, and PcVgR comprises two ligand-binding domains, two epidermal growth factor (EGF)-like regions containing YWTD motifs, a transmembrane domain, and a cytoplasmic domain. An analysis of the aa sequences and phylogenetics implied that both genes were genetically distinct from those of ticks and insects. The transcriptional profiles determined by real-time quantitative PCR in different developmental stages showed that both genes present the same expressional tendencies in eggs, larvae, nymphs, and...Continue Reading

References

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Citations

Feb 7, 2016·Parasitology Research·Geovane Dias-LopesJose Batista De Jesus
May 10, 2016·PloS One·Santosh Kumar UpadhyayPraveen C Verma
Apr 5, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Chaoshan HanQingyou Xia

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Datasets Mentioned

BETA
KC978893
KC978894

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

ExPASy Prosite SCAN
BLAST
SPSS
DNAMAN
BlastP
SignalP
MEGA5
ExPASy Molecular Biology Server
TMHMM

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