Molecular characterization, recombinant expression and bioactivity analysis of the interleukin-1 beta from the yellowfin sea bream, Acanthopagrus latus (Houttuyn)

Fish & Shellfish Immunology
Shigui JiangZhenxing Liu

Abstract

Interleukin-1 beta (IL-1 beta) is an important inflammatory mediator and has also the potential as an immunoadjuvant. Here we describe the isolation and characterization of yellowfin sea bream IL-1 beta (sbIL-1 beta) cDNA and gene. The sbIL-1 beta cDNA contains a 121-bp 5' untranslated region (UTR), a single open reading frame (ORF) of 762bp that translated into a 253 amino acid protein, a 342-bp 3' UTR with six cytokine RNA instability motifs (ATTTA), and a polyadenylation signal (AATAAA) at 13 nucleotides upstream of the poly (A) tail. The organization of the genomic IL-1 beta appears to be five exons and four introns, the intron and exon boundaries all follow the GT-AG consensus. The analysis of the expression pattern showed that sbIL-1 beta was expressed weakly in the kidney, spleen, gill and intestine, but not in the liver, heart and muscle. After injection with 150 microg LPS, the expression analysis in vivo showed that sbIL-1 beta was induced in the kidney and spleen and expression level was maximal after 4h. The predicted 253 amino acid sequence shares 23.5-88.5% identity and 43.9-93.3% similarity to known IL-1 beta. Thus, IL-1 beta is very conserved in fish of the same family. No interleukin-converting enzyme (ICE) cut...Continue Reading

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Citations

Dec 12, 2012·PloS One·Marta I R ReisNuno M S Dos Santos
Dec 4, 2012·International Journal of Molecular Sciences·Lei GaoWeidong Liu
Feb 23, 2013·Fish & Shellfish Immunology·Xin-Jiang LuYu-Hong Shi
Aug 15, 2012·Developmental and Comparative Immunology·Mansourah HusainTiehui Wang
Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
Mar 23, 2012·Veterinary Immunology and Immunopathology·Yan YuQuanqi Zhang
May 25, 2011·Developmental and Comparative Immunology·C J SecombesS Bird
Aug 21, 2010·Fish & Shellfish Immunology·Kevin Maisey, Mónica Imarai
Apr 14, 2009·General and Comparative Endocrinology·Dianchang ZhangXinping Xu
May 28, 2016·Biology·Jun Zou, Christopher J Secombes

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