Molecular Characterization Reveals Diverse and Unknown Malaria Vectors in the Western Kenyan Highlands

The American Journal of Tropical Medicine and Hygiene
Brandyce St LaurentJennifer Stevenson

Abstract

The success of mosquito-based malaria control is dependent upon susceptible bionomic traits in local malaria vectors. It is crucial to have accurate and reliable methods to determine mosquito species composition in areas subject to malaria. An unexpectedly diverse set of Anopheles species was collected in the western Kenyan highlands, including unidentified and potentially new species carrying the malaria parasite Plasmodium falciparum. This study identified 2,340 anopheline specimens using both ribosomal DNA internal transcribed spacer region 2 and mitochondrial DNA cytochrome oxidase subunit 1 loci. Seventeen distinct sequence groups were identified. Of these, only eight could be molecularly identified through comparison to published and voucher sequences. Of the unidentified species, four were found to carry P. falciparum by circumsporozoite enzyme-linked immunosorbent assay and polymerase chain reaction, the most abundant of which had infection rates comparable to a primary vector in the area, Anopheles funestus. High-quality adult specimens of these unidentified species could not be matched to museum voucher specimens or conclusively identified using multiple keys, suggesting that they may have not been previously describe...Continue Reading

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Citations

Dec 28, 2016·Insects·Jennifer C Stevenson, Douglas E Norris
Jan 21, 2016·The American Journal of Tropical Medicine and Hygiene·Jan E Conn
Mar 23, 2018·Parasitology·Nigel W Beebe
Dec 13, 2018·Wellcome Open Research·Claire L JeffriesThomas Walker
Nov 8, 2018·Parasites & Vectors·Edwin O OgolaDavid P Tchouassi
Feb 13, 2019·Parasites & Vectors·Edwin O OgolaDavid P Tchouassi
Feb 26, 2020·Malaria Journal·Yael Dahan-MossLizette L Koekemoer
Aug 8, 2018·Transactions of the Royal Society of Tropical Medicine and Hygiene·Janvier BandibaboneSeth R Irish
Jan 10, 2018·Malaria Journal·Brandyce St LaurentNeil F Lobo
Dec 18, 2019·International Journal of Environmental Research and Public Health·Sucharita GopalLawrence Were
Feb 12, 2021·Insects·Jordan E Hoffman On Behalf Of Southern And Central Africa International Centers Of Excellence For Malaria Research
Jan 19, 2021·Mitochondrial DNA. Part B, Resources·Melina CamposGregory C Lanzaro
Apr 10, 2021·Nature Reviews. Genetics·Daniel E NeafseyBronwyn L MacInnis

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