Abstract
Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on the pathogen's occurrence in other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. difficile isolates stemming from hospitalised patients with diarrhoea in Lebanon. From 129 stool samples of symptomatic patients at a tertiary care University hospital in Lebanon, a total of 107 C. difficile strains were cultivated and underwent ribotyping, toxin gene detection and antibiotic resistance testing. Ribotype 014 (RT014, 16.8%) predominated, followed by RT002 (9.3%), RT106 (8.4%) and RT070 (6.5%). Binary toxin gene-positive isolates (RT023, RT078 and RT126) were rarely detected and RT027 was absent. Interestingly, within one isolate only the toxin A gene (tcdA) was detected. Multiple-locus variable-number tandem repeat analysis (MLVA) revealed strong strain diversity in most RTs. The isolates were sensitive to metronidazole and vancomycin, and only a small proportion of strains displayed resistance against moxifloxacin, rifampicin, and clarithromycin (5.6%, 1.9%, and 2.8%), respectively. The data indicate that the genetic strain composition ...Continue Reading
References
Sep 24, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Abraham GoorhuisEd J Kuijper
Jun 17, 2009·Nature Reviews. Microbiology·Maja RupnikDale N Gerding
Mar 31, 2010·Emerging Infectious Diseases·Michael R MulveyUNKNOWN Canadian Nosocomial Infection Surveillance Program
Jul 9, 2010·Clinical Microbiology Reviews·J FreemanM H Wilcox
Sep 21, 2010·Anaerobe·Haihui HuangCarl Erik Nord
Jan 26, 2011·Antimicrobial Agents and Chemotherapy·Yi-Chun LinPo-Ren Hsueh
Mar 11, 2011·Journal of Clinical Microbiology·Fred C TenoverDavid H Persing
Apr 7, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V C C ChengK Y Yuen
Mar 8, 2012·PloS One·Richard A StablerBrendan W Wren
Mar 14, 2012·Antimicrobial Agents and Chemotherapy·Fred C TenoverDavid H Persing
Jun 21, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J KimW Fawley
Nov 2, 2012·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·L von MüllerM Herrmann
Dec 4, 2012·The Journal of Infectious Diseases·Kate E DingleDerrick W Crook
Dec 12, 2012·Nature Genetics·Miao HeTrevor D Lawley
Nov 21, 2013·Gut Microbes·Dale N GerdingKlaus Aktories
Feb 18, 2015·Epidemiology and Infection·C AguayoJ Fernández
Jun 28, 2015·Diagnostic Microbiology and Infectious Disease·Amos AdlerMitchell J Schwaber
Jul 30, 2015·The Journal of Antimicrobial Chemotherapy·Daniel R KnightThomas V Riley
Aug 1, 2015·Journal of Infection in Developing Countries·Romy MoukhaiberGhassan M Matar
Sep 12, 2015·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sören L BeckerLutz von Müller
Oct 9, 2015·Scientific Reports·Marc MonotBruno Dupuy
Jan 1, 2015·Antibiotics·Simon D Baines, Mark H Wilcox
Jul 30, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Kerrie A DaviesUNKNOWN EUCLID study group
Aug 19, 2016·PloS One·Wafaa Y Jamal, Vincent O Rotimi
Nov 20, 2016·PloS One·Yuan-Pin HungWen-Chien Ko
Jan 24, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Faten AlhomoudFarah Kais Alhomoud
Feb 28, 2017·Antimicrobial Resistance and Infection Control·Jacqueline FärberGernot Geginat
Citations
Aug 26, 2018·Letters in Applied Microbiology·S A BurtC Harmanus
Feb 26, 2019·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ebrahim KouhsariNour Amirmozafari
Aug 1, 2019·PloS One·Laura Tijerina-RodríguezElvira Garza-González
Apr 2, 2020·Scientific Reports·Reem Al AssaadMazen El Sayed
Jun 11, 2020·Emerging Microbes & Infections·Masoumeh AzimiradMohammad Reza Zali
Sep 27, 2020·Antimicrobial Resistance and Infection Control·Mohammad SholehEbrahim Kouhsari
Aug 23, 2018·Frontiers in Microbiology·Mwanaisha SeugendoUwe Groß
Sep 5, 2020·Indian Journal of Medical Microbiology·Taisir SaberEmad M Eed
Aug 15, 2020·Frontiers in Microbiology·Edyta WakerJerzy Ostrowski
Sep 26, 2019·Frontiers in Microbiology·Matthias F EmeleAndreas E Zautner
Feb 17, 2021·Indian Journal of Medical Microbiology·Taisir SaberEmad M Eed
Dec 17, 2020·Open Forum Infectious Diseases·Anna BerkefeldKathrin Rothe
Apr 30, 2021·International Journal of Medical Microbiology : IJMM·Ahmed Mohamed Mostafa AbdrabouFabian K Berger