Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon

International Journal of Medical Microbiology : IJMM
Fabian K BergerBarbara Gärtner

Abstract

Clostridium (Clostridioides) difficile is the main cause for nosocomial diarrhoea in industrialised nations. Epidemiologic data on the pathogen's occurrence in other world regions are still scarce. In this context we characterized with phenotypic and molecular genetic methods C. difficile isolates stemming from hospitalised patients with diarrhoea in Lebanon. From 129 stool samples of symptomatic patients at a tertiary care University hospital in Lebanon, a total of 107 C. difficile strains were cultivated and underwent ribotyping, toxin gene detection and antibiotic resistance testing. Ribotype 014 (RT014, 16.8%) predominated, followed by RT002 (9.3%), RT106 (8.4%) and RT070 (6.5%). Binary toxin gene-positive isolates (RT023, RT078 and RT126) were rarely detected and RT027 was absent. Interestingly, within one isolate only the toxin A gene (tcdA) was detected. Multiple-locus variable-number tandem repeat analysis (MLVA) revealed strong strain diversity in most RTs. The isolates were sensitive to metronidazole and vancomycin, and only a small proportion of strains displayed resistance against moxifloxacin, rifampicin, and clarithromycin (5.6%, 1.9%, and 2.8%), respectively. The data indicate that the genetic strain composition ...Continue Reading

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Citations

Feb 26, 2019·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ebrahim KouhsariNour Amirmozafari
Sep 27, 2020·Antimicrobial Resistance and Infection Control·Mohammad SholehEbrahim Kouhsari
Dec 17, 2020·Open Forum Infectious Diseases·Anna BerkefeldKathrin Rothe
Apr 30, 2021·International Journal of Medical Microbiology : IJMM·Ahmed Mohamed Mostafa AbdrabouFabian K Berger

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