Molecular classification of enteroviruses not identified by neutralization tests

Emerging Infectious Diseases
Hideyuki KuboYoshiyuki Seto

Abstract

We isolated six viruses from patients diagnosed with aseptic meningitis or hand, foot, and mouth disease. The cytopathic effect of these viruses on cultured cells was like that of enteroviruses. However, viral neutralization tests against standard antisera were negative. Phylogenetic analysis with the complete VP4 nucleotide sequences of these 6 viruses and 29 serotypes of enteroviruses classified 3 of the viruses as serotype echovirus type 18 (EV18) and 3 as serotype human enterovirus 71 (HEV71). These results were confirmed by remicroneutralization tests with HEV-monospecific antisera or an additional phylogenetic analysis with the complete VP4 nucleotide sequences. Phylogenetic analysis with complete VP4 genes is more useful than neutralization tests with enterovirus serotype-specific antisera in identifying enterovirus serotypes.

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Citations

Jul 14, 2011·BMC Musculoskeletal Disorders·In-Hwan OhYoung Ae Kim
Jun 30, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·David KiangDavid P Schnurr
Apr 21, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Pei-Yu ChuKuei-Hsiang Lin
Nov 27, 2018·Pathogens and Global Health·Olubusuyi Moses AdewumiJohnson Adekunle Adeniji
Feb 6, 2007·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Cristina E CabreraJames S Reilly
Apr 19, 2005·Journal of Medical Virology·Leighton E ClancyWilliam D Rawlinson
Feb 6, 2009·Bulletin de la Société de pathologie exotique·S AkréM Dosso

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Datasets Mentioned

BETA
AF250874
AF117697
AF117698
AJ2296215
CAV21
D90457
E1387

Methods Mentioned

BETA
PCR

Software Mentioned

CLUSTAL X

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