Molecular classification of liver cirrhosis in a rat model by proteomics and bioinformatics

Proteomics
Xiu-Qin XuHon-Chiu Eastwood Leung

Abstract

Liver cirrhosis is a worldwide health problem. Reliable, noninvasive methods for early detection of liver cirrhosis are not available. Using a three-step approach, we classified sera from rats with liver cirrhosis following different treatment insults. The approach consisted of: (i) protein profiling using surface-enhanced laser desorption/ionization (SELDI) technology; (ii) selection of a statistically significant serum biomarker set using machine learning algorithms; and (iii) identification of selected serum biomarkers by peptide sequencing. We generated serum protein profiles from three groups of rats: (i) normal (n=8), (ii) thioacetamide-induced liver cirrhosis (n=22), and (iii) bile duct ligation-induced liver fibrosis (n=5) using a weak cation exchanger surface. Profiling data were further analyzed by a recursive support vector machine algorithm to select a panel of statistically significant biomarkers for class prediction. Sensitivity and specificity of classification using the selected protein marker set were higher than 92%. A consistently down-regulated 3495 Da protein in cirrhosis samples was one of the selected significant biomarkers. This 3495 Da protein was purified on-chip and trypsin digested. Further structura...Continue Reading

Citations

Jan 3, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rihong ZhaiDavid Christiani
Dec 17, 2008·Gut·T AsselahP Marcellin
Nov 26, 2005·Expert Review of Proteomics·Eric T FungFujun Zhang
Mar 23, 2010·Trends in Molecular Medicine·Matthew L CowanSanjeev Krishna
Mar 26, 2005·Chembiochem : a European Journal of Chemical Biology·Kin-ya TomizakiHisakazu Mihara
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marc G Ghany, Edward Doo
Aug 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zobair M YounossiVikas Chandhoke
May 23, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Rosângela TeixeiraScott L Friedman
May 17, 2005·Journal of Hepatology·Romain Parent, Laura Beretta
Jan 9, 2009·Acta Biochimica Et Biophysica Sinica·Yunfei PeiXuegong Zhang
Jan 1, 2015·Microarrays·Amir SyahirHisakazu Mihara
May 24, 2008·Journal of Proteome Research·Daniela SpanoAchille Iolascon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
D B KristensenK Yoshizato
European Journal of Gastroenterology & Hepatology
Kin Jip CheungHans Van Vlierberghe
© 2021 Meta ULC. All rights reserved