PMID: 11342221May 9, 2001Paper

Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene

Biochimica Et Biophysica Acta
H MachidaT Nakano

Abstract

We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element.

References

Jul 23, 1991·Biochimica Et Biophysica Acta·Y KitagawaM Nagao
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H MiyoshiM Ohki
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Dec 11, 1996·Biochimica Et Biophysica Acta·K NomotoK Kikuchi
Sep 12, 1997·Science·J E Darnell
Dec 22, 1999·The Journal of Biological Chemistry·J FengT Nakano

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Citations

Sep 12, 2009·Experimental Cell Research·Erin TwomeyMarianna Sikorska
Sep 9, 2005·Transgenic Research·Ryuji OkamotoTakeshi Nakano
Sep 1, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Sanchita BasuAaron Proweller
Feb 22, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Hwee TeohMichael E Ward
Oct 8, 2021·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masaaki ItoKaoru Dohi

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