Molecular cloning and analysis of the ptsHI operon in Lactobacillus sake.

Applied and Environmental Microbiology
R StentzM Zagorec

Abstract

The ptsH and ptsI genes of Lactobacillus sake, encoding the general enzymes of the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS), were cloned and sequenced. HPr (88 amino acids), encoded by ptsH, and enzyme I (574 amino acids), encoded by ptsI, are homologous to the corresponding known enzymes of other bacteria. Nucleotide sequence and mRNA analysis showed that the two genes are cotranscribed in a large transcript encoding both HPr and enzyme I. The transcription of ptsHI was shown to be independent of the carbon source. Four ptsI mutants were constructed by single-crossover recombination. For all mutants, growth on PTS carbohydrates was abolished. Surprisingly, the growth rates of mutants on ribose and arabinose, two carbohydrates which are not transported by the PTS, were accelerated. This unexpected phenotype suggests that the PTS negatively controls ribose and arabinose utilization in L. sake by a mechanism different from the regulation involving HPr described for other gram-positive bacteria.

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Citations

Jul 31, 2002·Applied and Environmental Microbiology·Marie-Christine Champomier-VergèsMonique Zagorec
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 3, 2004·Applied and Environmental Microbiology·Anika MarceauMarie-Christine Champomier-Vergès
Nov 11, 1999·FEMS Microbiology Letters·M C Champomier VergèsS D Ehrlich
Jan 22, 2013·Applied and Environmental Microbiology·Jamila Anba-MondoloniMarie-Christine Champomier-Vergès
Feb 8, 2002·Microbiology·Anne-Marie DudezMonique Zagorec
Feb 13, 2007·Current Microbiology·Kristine NaterstadLars Axelsson
Dec 14, 2001·Biochemical and Biophysical Research Communications·P KotrbaH Yukawa

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