Molecular cloning and biochemical characterization of three phosphoglycerate kinase isoforms from developing sunflower (Helianthus annuus L.) seeds

Phytochemistry
M Adrián Troncoso-PonceEnrique Martínez-Force

Abstract

Three cDNAs encoding different phosphoglycerate kinase (PGK, EC 2.7.2.3) isoforms, two cytosolic (HacPGK1 and HacPGK2) and one plastidic (HapPGK), were cloned and characterized from developing sunflower (Helianthus annuus L.) seeds. The expression profiles of these genes showed differences in heterotrophic tissues, such as developing seeds and roots, where HacPGK1 was predominant, while HapPGK was highly expressed in photosynthetic tissues. The cDNAs were expressed in Escherichia coli, and the corresponding proteins purified to electrophoretic homogeneity, using immobilized metal ion affinity chromatography, and biochemically characterized. Despite the high level of identity between sequences, the HacPGK1 isoform showed strong differences in terms of specific activity, temperature stability and pH sensitivity in comparison to HacPGK2 and HapPGK. A polyclonal immune serum was raised against the purified HacPGK1 isoform, which showed cross-immunoreactivity with the other PGK isoforms. This serum allowed the localization of high expression levels of PGK isozymes in embryo tissues.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 26, 1971·European Journal of Biochemistry·M Larsson-Raźnikiewicz, L Arvidsson
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K RobertsO Jardetzky
Feb 1, 1996·Plant Molecular Biology·D H BringloeJ W Bradbeer
Sep 30, 1998·Biochemistry·A N Szilagyi, M Vas
Mar 24, 1999·Trends in Biochemical Sciences·C J Jeffery
Dec 10, 1999·The Journal of Biological Chemistry·T OginoK Mizumoto
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 25, 2002·Journal of Experimental Botany·Hardy RolletschekHans Weber
Jan 17, 2003·Plant Physiology·Lionel M HillStephen Rawsthorne
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Mar 3, 2004·Journal of Experimental Botany·David C BriceJennifer A Littlechild
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Malcolm C. Drew
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·William C. Plaxton
Jul 13, 2004·Plant Biology·L BorisjukH Weber
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 9, 2007·The Plant Journal : for Cell and Molecular Biology·Ana P AlonsoYair Shachar-Hill
Oct 30, 2007·Biochimica Et Biophysica Acta·K H StrucksbergJ Fitter
Feb 12, 2009·The New Phytologist·Ljudmilla Borisjuk, Hardy Rolletschek
Oct 19, 2010·Plant Physiology and Biochemistry : PPB·M Adrián Troncoso-PonceEnrique Martínez-Force
Feb 26, 2011·The Journal of Biological Chemistry·Louiza ZerradMatthew W Bowler
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fabienne BourgisVincent Arondel
Aug 20, 2011·The Plant Journal : for Cell and Molecular Biology·Manuel A Troncoso-PonceJohn B Ohlrogge

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.