Molecular Cloning and Bioinformatics Analysis of DQA Gene from Mink (Neovison vison )

International Journal of Molecular Sciences
Zhaobin FanPeihong Jiang

Abstract

In the present study, we cloned, sequenced, and explored the structural and functional characteristics of the major histocompatibility complex (MHC)-DQA gene from mink (Neovison vison) for the first time. The full-length sequence of DQA gene was 1147-bp-long, contained a coding region of 768-bp, which was predicted to encoding 255 amino acid residues. The comparison between DQA from mink (Neovison vison) and other MHC-DQA molecules from different animal species showed that nucleotide and encoded amino acid sequences of the mink DQA gene exhibited high similarity with the ferret (Mustela pulourius furo). Phylogenetic analysis revealed that mink (Neovison vison) DQA is grouped with that of ferret (Mustela pulourius furo). The cloned sequence contained a 23-amino acid NH₂-terminal signal sequence with the signal peptide cutting site located in amino acids 23⁻24, and had three Asn-Xaa-Ser/Thr sequons. Three cysteine residues were also identified (Cys-85, Cys-121, and Cys-138). The 218 to 240 amino acids were predicted to be the transmembrane domains. The prediction of the secondary structure revealed three α-helixes and fourteen β-sheets in Neovison vison DQA protein, while random coil was a major pattern. In this study, the whole ...Continue Reading

References

Sep 1, 1996·Tissue Antigens·J L WagnerR Storb
Sep 27, 2002·American Journal of Reproductive Immunology : AJRI·Andreas ZieglerBarbara Uchanska-Ziegler
Aug 19, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Julie DeterNathalie Charbonnel
Mar 11, 2009·Veterinary Immunology and Immunopathology·Saket K NiranjanSubodh Kumar
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Jun 11, 2016·Veterinary Microbiology·Jingmin GuWenyu Han

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Methods Mentioned

BETA
electrophoresis
PCR
glycosylation
Reverse Transcription PCR

Software Mentioned

NetPhos
EditSeq (
Lasergene
Target P1
DNAStar
Mega
TMHMM
SMART
ProtParam
MODEL

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