Molecular cloning and biological characterization of novel antimicrobial peptides, pilosulin 3 and pilosulin 4, from a species of the Australian ant genus Myrmecia

Archives of Biochemistry and Biophysics
Hidetoshi InagakiTai Kubo

Abstract

Venom of an Australian ant species of the Myrmecia pilosula species complex (mss. name Myrmecia banksi Taylor) contains two major allergenic peptides, pilosulin 1 and pilosulin 2. To obtain novel cDNA clones that encode the pilosulin-related bioactive peptides, mRNA of another Myrmecia species was subjected to RT-PCR in which the forward primer corresponds to a nucleotide sequence in the leader sequences of pilosulin 1 and pilosulin 2. As a result, we isolated cDNA clones encoding the novel antimicrobial peptides pilosulin 3 and pilosulin 4. The nucleotide and the amino acid sequences of all four pilosulins have high homology except for the mature peptide coding regions. Synthetic pilosulin 3 and pilosulin 4 peptides displayed antimicrobial activity with histamine-releasing and low hemolytic activities.

References

Mar 1, 1989·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A M Irani, L B Schwartz
Jul 22, 1998·Biochimie·S TaguchiJ A Hoffmann
Jul 30, 1999·Developmental and Comparative Immunology·P BuletD Hoffmann
Mar 30, 2001·The Journal of Biological Chemistry·J OrivelJ Rossier
Jan 25, 2002·Nature·Michael Zasloff
Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Forbes McGain, Kenneth D Winkel
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Noel W DaviesSimon G A Brown

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Citations

Sep 6, 2006·Die Naturwissenschaften·Rebeca B RosengausJames F A Traniello
Mar 27, 2012·PloS One·Christian W Gruber, Markus Muttenthaler
Jan 26, 2016·Toxins·Axel TouchardAlain Dejean
Oct 18, 2012·Journal of Insect Physiology·Sébastien J M Moreau
May 15, 2010·Toxicon : Official Journal of the International Society on Toxinology·Hidetoshi InagakiTai Kubo
Apr 10, 2010·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Richard W Weber
Dec 8, 2015·Molecular Immunology·Hathairat SrisongAndreas L Lopata
Dec 27, 2008·Toxicon : Official Journal of the International Society on Toxinology·Reto StöcklinDietrich Mebs
Apr 18, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuka NakajimaTai Kubo
Sep 10, 2015·Rapid Communications in Mass Spectrometry : RCM·Axel TouchardPierre Escoubas
Dec 27, 2005·Toxicon : Official Journal of the International Society on Toxinology·Michael D WieseRobert J Heddle
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Samira R AiliGraham M Nicholson
Sep 16, 2014·Biochimie·Kirill A PluzhnikovEugene V Grishin
Oct 26, 2011·Toxicon : Official Journal of the International Society on Toxinology·Hidetoshi InagakiTai Kubo
Mar 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·Troy WanandyMichael D Wiese
Oct 4, 2014·Trends in Ecology & Evolution·Oliver OttiHeike Feldhaar
Apr 16, 2013·Toxicon : Official Journal of the International Society on Toxinology·Wafa BouzidAngélique Vétillard
Dec 28, 2016·Toxins·Diana Aline Morais Ferreira NôgaAlessandra Mussi Ribeiro
Mar 28, 2017·Archives of Insect Biochemistry and Physiology·Nagachaitanya BhagavathulaChandrashekara Krishnappa
May 27, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Hiroko KawakamiTetsuro Shinada
May 2, 2018·Toxicon : Official Journal of the International Society on Toxinology·José Roberto Aparecido Dos Santos-PintoMario Sergio Palma
Aug 29, 2018·Journal of Proteome Research·Axel TouchardElsa Bonnafé

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